Validation of a new three-color fluorescence in situ hybridization (FISH) method to detect CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocations

Stephanie R. Fink, Kimberly J. Belongie, Sarah F. Paternoster, Stephanie A. Smoley, Animesh D. Pardanani, Ayalew Tefferi, Daniel L. Van Dyke, Rhett P. Ketterling

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The PDGRFA locus has become a gene of interest based on mutational activation in various myeloid neoplasms and the availability of targeted therapies (i.e., imatinib mesylate) to its overexpression. We studied a new FISH method to detect CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocation in patients with myeloid neoplasms associated with eosinophilia. A total of 46 specimens were studied, including 15 from patients with a CHIC2 abnormality and six patients with an abnormality involving PDGFRA. Our results revealed this new FISH assay accurately detects these abnormalities and will be a useful clinical test for patients with myeloid neoplasms and eosinophilia.

Original languageEnglish (US)
Pages (from-to)843-846
Number of pages4
JournalLeukemia Research
Volume33
Issue number6
DOIs
StatePublished - Jun 1 2009

Keywords

  • CHIC2
  • FIP1L1/PDGFRA fusion
  • FISH
  • Myeloproliferative neoplasm

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Validation of a new three-color fluorescence in situ hybridization (FISH) method to detect CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocations'. Together they form a unique fingerprint.

  • Cite this