Validation of a new three-color fluorescence in situ hybridization (FISH) method to detect CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocations

Stephanie R. Fink, Kimberly J. Belongie, Sarah F. Paternoster, Stephanie A. Smoley, Animesh D Pardanani, Ayalew Tefferi, Daniel L. Van Dyke, Rhett P. Ketterling

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The PDGRFA locus has become a gene of interest based on mutational activation in various myeloid neoplasms and the availability of targeted therapies (i.e., imatinib mesylate) to its overexpression. We studied a new FISH method to detect CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocation in patients with myeloid neoplasms associated with eosinophilia. A total of 46 specimens were studied, including 15 from patients with a CHIC2 abnormality and six patients with an abnormality involving PDGFRA. Our results revealed this new FISH assay accurately detects these abnormalities and will be a useful clinical test for patients with myeloid neoplasms and eosinophilia.

Original languageEnglish (US)
Pages (from-to)843-846
Number of pages4
JournalLeukemia Research
Volume33
Issue number6
DOIs
StatePublished - Jun 2009

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Keywords

  • CHIC2
  • FIP1L1/PDGFRA fusion
  • FISH
  • Myeloproliferative neoplasm

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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