Validation of a new three-color fluorescence in situ hybridization (FISH) method to detect CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocations

Stephanie R. Fink; Kimberly J. Belongie; Sarah F. Paternoster; Stephanie A. Smoley; Animesh D. Pardanani; Ayalew Tefferi; Daniel L. Van Dyke; Rhett P. Ketterling

doi:10.1016/j.leukres.2008.11.016

Validation of a new three-color fluorescence in situ hybridization (FISH) method to detect CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocations

Stephanie R. Fink, Kimberly J. Belongie, Sarah F. Paternoster, Stephanie A. Smoley, Animesh D. Pardanani, Ayalew Tefferi, Daniel L. Van Dyke, Rhett P. Ketterling

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The PDGRFA locus has become a gene of interest based on mutational activation in various myeloid neoplasms and the availability of targeted therapies (i.e., imatinib mesylate) to its overexpression. We studied a new FISH method to detect CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocation in patients with myeloid neoplasms associated with eosinophilia. A total of 46 specimens were studied, including 15 from patients with a CHIC2 abnormality and six patients with an abnormality involving PDGFRA. Our results revealed this new FISH assay accurately detects these abnormalities and will be a useful clinical test for patients with myeloid neoplasms and eosinophilia.

Original language	English (US)
Pages (from-to)	843-846
Number of pages	4
Journal	Leukemia Research
Volume	33
Issue number	6
DOIs	https://doi.org/10.1016/j.leukres.2008.11.016
State	Published - Jun 1 2009

Keywords

CHIC2
FIP1L1/PDGFRA fusion
FISH
Myeloproliferative neoplasm

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

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10.1016/j.leukres.2008.11.016

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Fink, S. R., Belongie, K. J., Paternoster, S. F., Smoley, S. A., Pardanani, A. D., Tefferi, A., Van Dyke, D. L., & Ketterling, R. P. (2009). Validation of a new three-color fluorescence in situ hybridization (FISH) method to detect CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocations. Leukemia Research, 33(6), 843-846. https://doi.org/10.1016/j.leukres.2008.11.016

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title = "Validation of a new three-color fluorescence in situ hybridization (FISH) method to detect CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocations",

abstract = "The PDGRFA locus has become a gene of interest based on mutational activation in various myeloid neoplasms and the availability of targeted therapies (i.e., imatinib mesylate) to its overexpression. We studied a new FISH method to detect CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocation in patients with myeloid neoplasms associated with eosinophilia. A total of 46 specimens were studied, including 15 from patients with a CHIC2 abnormality and six patients with an abnormality involving PDGFRA. Our results revealed this new FISH assay accurately detects these abnormalities and will be a useful clinical test for patients with myeloid neoplasms and eosinophilia.",

keywords = "CHIC2, FIP1L1/PDGFRA fusion, FISH, Myeloproliferative neoplasm",

author = "Fink, {Stephanie R.} and Belongie, {Kimberly J.} and Paternoster, {Sarah F.} and Smoley, {Stephanie A.} and Pardanani, {Animesh D.} and Ayalew Tefferi and {Van Dyke}, {Daniel L.} and Ketterling, {Rhett P.}",

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AU - Fink, Stephanie R.

AU - Belongie, Kimberly J.

AU - Paternoster, Sarah F.

AU - Smoley, Stephanie A.

AU - Pardanani, Animesh D.

AU - Tefferi, Ayalew

AU - Van Dyke, Daniel L.

AU - Ketterling, Rhett P.

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AB - The PDGRFA locus has become a gene of interest based on mutational activation in various myeloid neoplasms and the availability of targeted therapies (i.e., imatinib mesylate) to its overexpression. We studied a new FISH method to detect CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocation in patients with myeloid neoplasms associated with eosinophilia. A total of 46 specimens were studied, including 15 from patients with a CHIC2 abnormality and six patients with an abnormality involving PDGFRA. Our results revealed this new FISH assay accurately detects these abnormalities and will be a useful clinical test for patients with myeloid neoplasms and eosinophilia.

KW - CHIC2

KW - FIP1L1/PDGFRA fusion

KW - FISH

KW - Myeloproliferative neoplasm

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Validation of a new three-color fluorescence in situ hybridization (FISH) method to detect CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocations

Abstract

Keywords

ASJC Scopus subject areas

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