Validation of a new three-color fluorescence in situ hybridization (FISH) method to detect CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocations

Stephanie R. Fink, Kimberly J. Belongie, Sarah F. Paternoster, Stephanie A. Smoley, Animesh D Pardanani, Ayalew Tefferi, Daniel L. Van Dyke, Rhett P. Ketterling

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The PDGRFA locus has become a gene of interest based on mutational activation in various myeloid neoplasms and the availability of targeted therapies (i.e., imatinib mesylate) to its overexpression. We studied a new FISH method to detect CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocation in patients with myeloid neoplasms associated with eosinophilia. A total of 46 specimens were studied, including 15 from patients with a CHIC2 abnormality and six patients with an abnormality involving PDGFRA. Our results revealed this new FISH assay accurately detects these abnormalities and will be a useful clinical test for patients with myeloid neoplasms and eosinophilia.

Original languageEnglish (US)
Pages (from-to)843-846
Number of pages4
JournalLeukemia Research
Volume33
Issue number6
DOIs
StatePublished - Jun 2009

Fingerprint

Fluorescence In Situ Hybridization
Color
Eosinophilia
Neoplasms
Genes
Therapeutics

Keywords

  • CHIC2
  • FIP1L1/PDGFRA fusion
  • FISH
  • Myeloproliferative neoplasm

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Validation of a new three-color fluorescence in situ hybridization (FISH) method to detect CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocations. / Fink, Stephanie R.; Belongie, Kimberly J.; Paternoster, Sarah F.; Smoley, Stephanie A.; Pardanani, Animesh D; Tefferi, Ayalew; Van Dyke, Daniel L.; Ketterling, Rhett P.

In: Leukemia Research, Vol. 33, No. 6, 06.2009, p. 843-846.

Research output: Contribution to journalArticle

Fink, Stephanie R. ; Belongie, Kimberly J. ; Paternoster, Sarah F. ; Smoley, Stephanie A. ; Pardanani, Animesh D ; Tefferi, Ayalew ; Van Dyke, Daniel L. ; Ketterling, Rhett P. / Validation of a new three-color fluorescence in situ hybridization (FISH) method to detect CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocations. In: Leukemia Research. 2009 ; Vol. 33, No. 6. pp. 843-846.
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