Utilizing magnetization transfer imaging to investigate tissue remodeling in a murine model of autosomal dominant polycystic kidney disease

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Abstract

Purpose: Noninvasive imaging techniques that quantify renal tissue composition are needed to more accurately ascertain prognosis and monitor disease progression in polycystic kidney disease (PKD). Given the success of magnetization transfer (MT) imaging to characterize various tissue remodeling pathologies, it was tested on a murine model of autosomal dominant PKD. Methods: C57Bl/6 Pkd1 R3277C mice at 9, 12, and 15 months were imaged with a 16.4T MR imaging system. Images were acquired without and with RF saturation in order to calculate MT ratio (MTR) maps. Following imaging, the mice were euthanized and kidney sections were analyzed for cystic and fibrotic indices, which were compared with statistical parameters of the MTR maps. Results: The MTR-derived mean, median, 25th percentile, skewness, and kurtosis were all closely related to indices of renal pathology, including kidney weight/body weight, cystic index, and percent of remaining parenchyma. The correlation between MTR and histology-derived cystic and fibrotic changes was R<sup>2</sup>=0.84 and R<sup>2</sup>=0.70, respectively. Conclusion: MT imaging provides a new, noninvasive means of measuring tissue remodeling PKD changes and may be better suited for characterizing renal impairment compared with conventional MR techniques.

Original languageEnglish (US)
JournalMagnetic Resonance in Medicine
DOIs
StateAccepted/In press - 2015

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Autosomal Dominant Polycystic Kidney
Kidney
Polycystic Kidney Diseases
Pathology
Disease Progression
Histology
Body Weight
Weights and Measures

Keywords

  • Fibrosis
  • Gaussian mixture model
  • Histology
  • In vivo imaging
  • Quantitative MRI
  • Skewness

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

@article{a15e9151589d49c78481c5fe22373f13,
title = "Utilizing magnetization transfer imaging to investigate tissue remodeling in a murine model of autosomal dominant polycystic kidney disease",
abstract = "Purpose: Noninvasive imaging techniques that quantify renal tissue composition are needed to more accurately ascertain prognosis and monitor disease progression in polycystic kidney disease (PKD). Given the success of magnetization transfer (MT) imaging to characterize various tissue remodeling pathologies, it was tested on a murine model of autosomal dominant PKD. Methods: C57Bl/6 Pkd1 R3277C mice at 9, 12, and 15 months were imaged with a 16.4T MR imaging system. Images were acquired without and with RF saturation in order to calculate MT ratio (MTR) maps. Following imaging, the mice were euthanized and kidney sections were analyzed for cystic and fibrotic indices, which were compared with statistical parameters of the MTR maps. Results: The MTR-derived mean, median, 25th percentile, skewness, and kurtosis were all closely related to indices of renal pathology, including kidney weight/body weight, cystic index, and percent of remaining parenchyma. The correlation between MTR and histology-derived cystic and fibrotic changes was R2=0.84 and R2=0.70, respectively. Conclusion: MT imaging provides a new, noninvasive means of measuring tissue remodeling PKD changes and may be better suited for characterizing renal impairment compared with conventional MR techniques.",
keywords = "Fibrosis, Gaussian mixture model, Histology, In vivo imaging, Quantitative MRI, Skewness",
author = "Timothy Kline and {Irazabal Mira}, Maria and Behzad Ebrahimi and Katharina Hopp and Udoji, {Kelly N.} and Warner, {Joshua D.} and Panagiotis Korfiatis and Mishra, {Prasanna K.} and Macura, {Slobodan I} and Venkatesh, {Sudhakar K} and Lerman, {Lilach O} and Harris, {Peter C} and Vicente Torres and King, {Bernard Francis} and Erickson, {Bradley J}",
year = "2015",
doi = "10.1002/mrm.25701",
language = "English (US)",
journal = "Magnetic Resonance in Medicine",
issn = "0740-3194",
publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Utilizing magnetization transfer imaging to investigate tissue remodeling in a murine model of autosomal dominant polycystic kidney disease

AU - Kline, Timothy

AU - Irazabal Mira, Maria

AU - Ebrahimi, Behzad

AU - Hopp, Katharina

AU - Udoji, Kelly N.

AU - Warner, Joshua D.

AU - Korfiatis, Panagiotis

AU - Mishra, Prasanna K.

AU - Macura, Slobodan I

AU - Venkatesh, Sudhakar K

AU - Lerman, Lilach O

AU - Harris, Peter C

AU - Torres, Vicente

AU - King, Bernard Francis

AU - Erickson, Bradley J

PY - 2015

Y1 - 2015

N2 - Purpose: Noninvasive imaging techniques that quantify renal tissue composition are needed to more accurately ascertain prognosis and monitor disease progression in polycystic kidney disease (PKD). Given the success of magnetization transfer (MT) imaging to characterize various tissue remodeling pathologies, it was tested on a murine model of autosomal dominant PKD. Methods: C57Bl/6 Pkd1 R3277C mice at 9, 12, and 15 months were imaged with a 16.4T MR imaging system. Images were acquired without and with RF saturation in order to calculate MT ratio (MTR) maps. Following imaging, the mice were euthanized and kidney sections were analyzed for cystic and fibrotic indices, which were compared with statistical parameters of the MTR maps. Results: The MTR-derived mean, median, 25th percentile, skewness, and kurtosis were all closely related to indices of renal pathology, including kidney weight/body weight, cystic index, and percent of remaining parenchyma. The correlation between MTR and histology-derived cystic and fibrotic changes was R2=0.84 and R2=0.70, respectively. Conclusion: MT imaging provides a new, noninvasive means of measuring tissue remodeling PKD changes and may be better suited for characterizing renal impairment compared with conventional MR techniques.

AB - Purpose: Noninvasive imaging techniques that quantify renal tissue composition are needed to more accurately ascertain prognosis and monitor disease progression in polycystic kidney disease (PKD). Given the success of magnetization transfer (MT) imaging to characterize various tissue remodeling pathologies, it was tested on a murine model of autosomal dominant PKD. Methods: C57Bl/6 Pkd1 R3277C mice at 9, 12, and 15 months were imaged with a 16.4T MR imaging system. Images were acquired without and with RF saturation in order to calculate MT ratio (MTR) maps. Following imaging, the mice were euthanized and kidney sections were analyzed for cystic and fibrotic indices, which were compared with statistical parameters of the MTR maps. Results: The MTR-derived mean, median, 25th percentile, skewness, and kurtosis were all closely related to indices of renal pathology, including kidney weight/body weight, cystic index, and percent of remaining parenchyma. The correlation between MTR and histology-derived cystic and fibrotic changes was R2=0.84 and R2=0.70, respectively. Conclusion: MT imaging provides a new, noninvasive means of measuring tissue remodeling PKD changes and may be better suited for characterizing renal impairment compared with conventional MR techniques.

KW - Fibrosis

KW - Gaussian mixture model

KW - Histology

KW - In vivo imaging

KW - Quantitative MRI

KW - Skewness

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