Utility of peripheral blood dual color, double fusion fluorescent in situ hybridization for BCR/ABL fusion to assess cytogenetic remission status in chronic myeloid leukemia

Andrew P. Landstrom, Rhett P. Ketterling, Ryan A. Knudson, Ayalew Tefferi

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The molecular signature of BCR-ABL fusion in chronic myeloid leukemia (CML) provides a unique tool for diagnosis and monitoring of tumor burden during therapy. The gold standard in this regard is conventional bone marrow cytogenetics. Peripheral blood fluorescent in situ hybridization (FISH) offers the possibility of a less invasive, more practical alternative method in terms of both cost and turnaround time. In the present study, we examined 296 paired samples from 65 patients with CML and demonstrate a tight correlation, in quantifying BCR-ABL burden between bone marrow cytogenetics, bone marrow dual color, double fusion (D-FISH), and peripheral blood D-FISH (P < 0.0001 for each). Furthermore, we demonstrate that peripheral blood D-FISH can be used as a surrogate for cytogenetic studies in monitoring cytogenetic remission status.

Original languageEnglish (US)
Pages (from-to)2055-2061
Number of pages7
JournalLeukemia and Lymphoma
Volume47
Issue number10
DOIs
StatePublished - Oct 1 2006

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Keywords

  • Chronic myeloid leukemia
  • Cytogenetics
  • FISH
  • Philadelphia chromosome

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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