Uterine leiomyomas express myometrial contractile-associated proteins involved in pregnancy-related hormone signaling

Kimberley Cesen-Cummings, Kevin D. Houston, John A III Copland, Valerie J. Moorman, Cheryl Lyn Walker, Barbara J. Davis

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Objective: We used an animal model to study uterine leiomyoma in the context of pregnancy-associated changes in gene expression and to determine how they might modulate tumor growth. Methods: Spontaneous tumors and normal myometrium were collected from Eker rats and compared with myometrial samples from pregnant animals. A leiomyoma-derived cell line was also used to assess pregnancy-related changes in gene expression and to determine the impact of signaling by the oxytocin receptor. Results: Eker rat leiomyomas expressed several pregnancy-related genes, including connexin 43, oxytocin receptor (OTR), and cyclooxygenase (COX)-1; however, the tumors did not express COX-2, which is expressed in the parturient myometrium. The leiomyoma-derived cell lines also expressed OTR, which responds to estrogen, binds to oxytocin, and exhibits a calcium flux when stimulated with oxytocin. The OTR signaling mediated by oxytocin inhibited estrogen-stimulated growth of leiomyoma cells. Conclusions: Leiomyoma cells expressed many genes of the parturient myometrium, including OTRs, but were deficient in COX-2 expression. Signaling via the OTR appears to inhibit estrogen-induced cell proliferation, suggesting that signaling by this receptor might help mediate the protective effect of pregnancy on this disease.

Original languageEnglish (US)
Pages (from-to)11-20
Number of pages10
JournalJournal of the Society for Gynecologic Investigation
Volume10
Issue number1
DOIs
StatePublished - Jan 2003
Externally publishedYes

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Contractile Proteins
Oxytocin Receptors
Leiomyoma
Hormones
Pregnancy
Myometrium
Oxytocin
Estrogens
Cyclooxygenase 2
Parturition
Gene Expression
Cell Line
Neoplasms
Cyclooxygenase 1
Connexin 43
Growth
Genes
Animal Models
Cell Proliferation
Calcium

Keywords

  • Leiomyoma
  • Myometrium
  • Oxytocin receptor
  • Parturition
  • Uterus

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Uterine leiomyomas express myometrial contractile-associated proteins involved in pregnancy-related hormone signaling. / Cesen-Cummings, Kimberley; Houston, Kevin D.; Copland, John A III; Moorman, Valerie J.; Walker, Cheryl Lyn; Davis, Barbara J.

In: Journal of the Society for Gynecologic Investigation, Vol. 10, No. 1, 01.2003, p. 11-20.

Research output: Contribution to journalArticle

Cesen-Cummings, Kimberley ; Houston, Kevin D. ; Copland, John A III ; Moorman, Valerie J. ; Walker, Cheryl Lyn ; Davis, Barbara J. / Uterine leiomyomas express myometrial contractile-associated proteins involved in pregnancy-related hormone signaling. In: Journal of the Society for Gynecologic Investigation. 2003 ; Vol. 10, No. 1. pp. 11-20.
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abstract = "Objective: We used an animal model to study uterine leiomyoma in the context of pregnancy-associated changes in gene expression and to determine how they might modulate tumor growth. Methods: Spontaneous tumors and normal myometrium were collected from Eker rats and compared with myometrial samples from pregnant animals. A leiomyoma-derived cell line was also used to assess pregnancy-related changes in gene expression and to determine the impact of signaling by the oxytocin receptor. Results: Eker rat leiomyomas expressed several pregnancy-related genes, including connexin 43, oxytocin receptor (OTR), and cyclooxygenase (COX)-1; however, the tumors did not express COX-2, which is expressed in the parturient myometrium. The leiomyoma-derived cell lines also expressed OTR, which responds to estrogen, binds to oxytocin, and exhibits a calcium flux when stimulated with oxytocin. The OTR signaling mediated by oxytocin inhibited estrogen-stimulated growth of leiomyoma cells. Conclusions: Leiomyoma cells expressed many genes of the parturient myometrium, including OTRs, but were deficient in COX-2 expression. Signaling via the OTR appears to inhibit estrogen-induced cell proliferation, suggesting that signaling by this receptor might help mediate the protective effect of pregnancy on this disease.",
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