Using mechanical homogenization to isolate microglia from mouse brain tissue to preserve transcriptomic integrity

Shawn Herron, Jean Christophe Delpech, Charlotte Madore, Tsuneya Ikezu

Research output: Contribution to journalArticlepeer-review

Abstract

Numerous approaches have been developed to isolate microglia from the brain, but procedures using enzymatic dissociation at 37°C can introduce drastic transcriptomic changes and confound results from gene expression assays. Here, we present an optimized protocol for microglia isolation using mechanical homogenization. We use Dounce homogenization to homogenize mouse brain tissue into single-cell suspension. We then isolate microglia through Percoll gradient and flow cytometry. Isolated microglia exhibit a gene expression pattern without the changes induced by heated enzymatic digestion. For complete details on the use and execution of this protocol, please refer to Clayton et al. (2021).

Original languageEnglish (US)
Article number101670
JournalSTAR Protocols
Volume3
Issue number4
DOIs
StatePublished - Dec 16 2022

Keywords

  • Cell isolation
  • Cell separation/fractionation
  • Classification Description: Cell biology
  • Flow cytometry/Mass cytometry
  • Gene expression
  • Molecular biology
  • Neuroscience
  • RNAseq

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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