TY - GEN
T1 - Using magnetic resonance microscopy to assess the osteogenesis in porous hydrogels
AU - Mishra, Prasanna
AU - Dadsetan, Mahrokh
AU - Rajagopalan, Srinivasan
AU - Hefferan, Theresa E.
AU - Yaszemski, Michael J.
AU - Macura, Slobodan I.
PY - 2007
Y1 - 2007
N2 - Hydrogels are multi-functional and can be used as scaffolds in bone tissue engineering. The pore architecture of the scaffolds is a significant factor in bone cell function. In this work, oligo (poly (ethylene glycol) fumarate) (OPF) hydrogel is used in the fabrication of porous scaffolds, and the effect of hydrogel porosity on bone formation is evaluated using an in vitro bone marrow stromal cell model. The porous hydrogels consist of copious amounts of water (90% or more by volume), and their structure is very similar to soft tissues. The characterization of porosity and interconnectivity in a typical OPF hydrogel scaffold and the bone formation inside the scaffold are assessed using magnetic resonance microscopy (MRM) techniques and are shown to be very useful in micro structural studies of such water rich materials. A Bruker Avance 7 Tesla (proton 300 MHz) spectrometer equipped with a Micro Imaging 2.5 accessory is used in these studies. By optimizing the acquisition parameters such as relaxation recovery time (TR), echo time (TE), pulse flip angle (TA), and using solvent relaxation-enhancing media, the pores and bone formations are clearly observed at an in-plane resolution of 29 μm/ pixel in various scaffold materials. Analysis of our MRM images shows that the pores are highly interconnected and the porosity computed from the images correlate quite well with the experimental porosity parameters such as porogen size and percentage. In addition, the quantitative alkaline phosphatase activity, alizarin red staining as marker of osteoblastic differentiation, and the mineralization capacity of marrow stromal cells are in good agreement with the bone formation seen in the MRM images.
AB - Hydrogels are multi-functional and can be used as scaffolds in bone tissue engineering. The pore architecture of the scaffolds is a significant factor in bone cell function. In this work, oligo (poly (ethylene glycol) fumarate) (OPF) hydrogel is used in the fabrication of porous scaffolds, and the effect of hydrogel porosity on bone formation is evaluated using an in vitro bone marrow stromal cell model. The porous hydrogels consist of copious amounts of water (90% or more by volume), and their structure is very similar to soft tissues. The characterization of porosity and interconnectivity in a typical OPF hydrogel scaffold and the bone formation inside the scaffold are assessed using magnetic resonance microscopy (MRM) techniques and are shown to be very useful in micro structural studies of such water rich materials. A Bruker Avance 7 Tesla (proton 300 MHz) spectrometer equipped with a Micro Imaging 2.5 accessory is used in these studies. By optimizing the acquisition parameters such as relaxation recovery time (TR), echo time (TE), pulse flip angle (TA), and using solvent relaxation-enhancing media, the pores and bone formations are clearly observed at an in-plane resolution of 29 μm/ pixel in various scaffold materials. Analysis of our MRM images shows that the pores are highly interconnected and the porosity computed from the images correlate quite well with the experimental porosity parameters such as porogen size and percentage. In addition, the quantitative alkaline phosphatase activity, alizarin red staining as marker of osteoblastic differentiation, and the mineralization capacity of marrow stromal cells are in good agreement with the bone formation seen in the MRM images.
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M3 - Conference contribution
AN - SCOPUS:41549095555
SN - 9781604234312
T3 - Materials Research Society Symposium Proceedings
SP - 33
EP - 38
BT - Magnetic Resonance in Material Science
T2 - 2006 MRS Fall Meeting
Y2 - 27 November 2006 through 1 December 2006
ER -