Using engineered endonucleases to create knockout and knockin zebrafish models

Victoria M. Bedell, Stephen C Ekker

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Over the last few years, the technology to create targeted knockout and knockin zebrafish animals has exploded. We have gained the ability to create targeted knockouts through the use of zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/CRISPR associated system (CRISPR/Cas). Furthermore, using the high-efficiency TALEN system, we were able to create knockin zebrafish using a single-stranded DNA (ssDNA) protocol described here. Through the use of these technologies, the zebrafish has become a valuable vertebrate model and an excellent bridge between the invertebrate and mammalian model systems for the study of human disease.

Original languageEnglish (US)
Pages (from-to)291-306
Number of pages16
JournalMethods in Molecular Biology
Volume1239
DOIs
StatePublished - 2015

Fingerprint

Endonucleases
Zebrafish
Clustered Regularly Interspaced Short Palindromic Repeats
Technology
Single-Stranded DNA
Zinc Fingers
Invertebrates
Vertebrates
Transcription Activator-Like Effector Nucleases

Keywords

  • Genome engineering
  • HDR
  • Homology directed repair
  • TALEN
  • Zebrafish

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Using engineered endonucleases to create knockout and knockin zebrafish models. / Bedell, Victoria M.; Ekker, Stephen C.

In: Methods in Molecular Biology, Vol. 1239, 2015, p. 291-306.

Research output: Contribution to journalArticle

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