Earlier attempts to purify the opioid receptors met with limited success because of the use of brain crude membranes. Subcellular fractionation procedures adopted now to get enriched membranes resulted in 2-3 fold enrichment. However, a procedure has been developed in our laboratory in which a crude membrane fraction obtained at 17,500 x g was lysed with 1 mM sodium bicarbonate and later subjected to zonal fractionation, employing a density gradient of 0.6-1.2 M sucrose. This could yield a membrane fraction highly enriched with μ-opioid receptors which is 9.3 fold higher than the crude membranes.
- Subcellular fractionation
- Zonal centrifugation
- μ-Opioid receptor
- μ-Opioid receptor rich membranes
ASJC Scopus subject areas