Use of zonal centrifugation method for the preparation of μ-opioid receptor enriched membranes from bovine corpus striatum

K. V.H. Sastry, K. G. Harikumar, P. Sailaja, M. K. Janardana Sarma

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Earlier attempts to purify the opioid receptors met with limited success because of the use of brain crude membranes. Subcellular fractionation procedures adopted now to get enriched membranes resulted in 2-3 fold enrichment. However, a procedure has been developed in our laboratory in which a crude membrane fraction obtained at 17,500 x g was lysed with 1 mM sodium bicarbonate and later subjected to zonal fractionation, employing a density gradient of 0.6-1.2 M sucrose. This could yield a membrane fraction highly enriched with μ-opioid receptors which is 9.3 fold higher than the crude membranes.

Original languageEnglish (US)
Pages (from-to)45-52
Number of pages8
JournalJournal of Neuroscience Methods
Volume74
Issue number1
DOIs
StatePublished - Jun 6 1997

Keywords

  • Subcellular fractionation
  • Zonal centrifugation
  • μ-Opioid receptor
  • μ-Opioid receptor rich membranes

ASJC Scopus subject areas

  • Neuroscience(all)

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