Use of ultra-high field MRI in small rodent models of polycystic kidney disease for in vivo phenotyping and drug monitoring

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Abstract

Several in vivo pre-clinical studies in Polycystic Kidney Disease (PKD) utilize orthologous rodent models to identify and study the genetic and molecular mechanisms responsible for the disease, and are very convenient for rapid drug screening and testing of promising therapies. A limiting factor in these studies is often the lack of efficient non-invasive methods for sequentially analyzing the anatomical and functional changes in the kidney. Magnetic resonance imaging (MRI) is the current gold standard imaging technique to follow autosomal dominant polycystic kidney disease (ADPKD) patients, providing excellent soft tissue contrast and anatomic detail and allowing Total Kidney Volume (TKV) measurements. A major advantage of MRI in rodent models of PKD is the possibility for in vivo imaging allowing for longitudinal studies that use the same animal and therefore reducing the total number of animals required. In this manuscript, we will focus on using Ultra-high field (UHF) MRI to noninvasively acquire in vivo images of rodent models for PKD. The main goal of this work is to introduce the use of MRI as a tool for in vivo phenotypical characterization and drug monitoring in rodent models for PKD.

Original languageEnglish (US)
Article numbere52757
JournalJournal of Visualized Experiments
Volume2015
Issue number100
DOIs
StatePublished - Jun 23 2015

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Polycystic Kidney Diseases
Drug Monitoring
Magnetic resonance imaging
Rodentia
Magnetic Resonance Imaging
Monitoring
Pharmaceutical Preparations
Kidney
Autosomal Dominant Polycystic Kidney
Preclinical Drug Evaluations
Animals
Longitudinal Studies
Imaging techniques
Volume measurement
Molecular Biology
Screening
Tissue
Testing
Therapeutics

Keywords

  • Autosomal dominant polycystic kidney disease (ADPKD)
  • Autosomal-recessive polycystic kidney disease (ARPKD)
  • Cysts
  • Interventions
  • Issue 100
  • Kidney
  • Magnetic resonance imaging (MRI)
  • Medicine
  • Phenotype
  • Polycystic kidney disease (PKD)
  • Progression
  • Rodent
  • Total kidney volume (TKV)
  • Ultra-high field (UHF) MRI

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)
  • Medicine(all)

Cite this

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title = "Use of ultra-high field MRI in small rodent models of polycystic kidney disease for in vivo phenotyping and drug monitoring",
abstract = "Several in vivo pre-clinical studies in Polycystic Kidney Disease (PKD) utilize orthologous rodent models to identify and study the genetic and molecular mechanisms responsible for the disease, and are very convenient for rapid drug screening and testing of promising therapies. A limiting factor in these studies is often the lack of efficient non-invasive methods for sequentially analyzing the anatomical and functional changes in the kidney. Magnetic resonance imaging (MRI) is the current gold standard imaging technique to follow autosomal dominant polycystic kidney disease (ADPKD) patients, providing excellent soft tissue contrast and anatomic detail and allowing Total Kidney Volume (TKV) measurements. A major advantage of MRI in rodent models of PKD is the possibility for in vivo imaging allowing for longitudinal studies that use the same animal and therefore reducing the total number of animals required. In this manuscript, we will focus on using Ultra-high field (UHF) MRI to noninvasively acquire in vivo images of rodent models for PKD. The main goal of this work is to introduce the use of MRI as a tool for in vivo phenotypical characterization and drug monitoring in rodent models for PKD.",
keywords = "Autosomal dominant polycystic kidney disease (ADPKD), Autosomal-recessive polycystic kidney disease (ARPKD), Cysts, Interventions, Issue 100, Kidney, Magnetic resonance imaging (MRI), Medicine, Phenotype, Polycystic kidney disease (PKD), Progression, Rodent, Total kidney volume (TKV), Ultra-high field (UHF) MRI",
author = "{Irazabal Mira}, Maria and Mishra, {Prasanna K.} and Vicente Torres and Macura, {Slobodan I}",
year = "2015",
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doi = "10.3791/52757",
language = "English (US)",
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AU - Irazabal Mira, Maria

AU - Mishra, Prasanna K.

AU - Torres, Vicente

AU - Macura, Slobodan I

PY - 2015/6/23

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N2 - Several in vivo pre-clinical studies in Polycystic Kidney Disease (PKD) utilize orthologous rodent models to identify and study the genetic and molecular mechanisms responsible for the disease, and are very convenient for rapid drug screening and testing of promising therapies. A limiting factor in these studies is often the lack of efficient non-invasive methods for sequentially analyzing the anatomical and functional changes in the kidney. Magnetic resonance imaging (MRI) is the current gold standard imaging technique to follow autosomal dominant polycystic kidney disease (ADPKD) patients, providing excellent soft tissue contrast and anatomic detail and allowing Total Kidney Volume (TKV) measurements. A major advantage of MRI in rodent models of PKD is the possibility for in vivo imaging allowing for longitudinal studies that use the same animal and therefore reducing the total number of animals required. In this manuscript, we will focus on using Ultra-high field (UHF) MRI to noninvasively acquire in vivo images of rodent models for PKD. The main goal of this work is to introduce the use of MRI as a tool for in vivo phenotypical characterization and drug monitoring in rodent models for PKD.

AB - Several in vivo pre-clinical studies in Polycystic Kidney Disease (PKD) utilize orthologous rodent models to identify and study the genetic and molecular mechanisms responsible for the disease, and are very convenient for rapid drug screening and testing of promising therapies. A limiting factor in these studies is often the lack of efficient non-invasive methods for sequentially analyzing the anatomical and functional changes in the kidney. Magnetic resonance imaging (MRI) is the current gold standard imaging technique to follow autosomal dominant polycystic kidney disease (ADPKD) patients, providing excellent soft tissue contrast and anatomic detail and allowing Total Kidney Volume (TKV) measurements. A major advantage of MRI in rodent models of PKD is the possibility for in vivo imaging allowing for longitudinal studies that use the same animal and therefore reducing the total number of animals required. In this manuscript, we will focus on using Ultra-high field (UHF) MRI to noninvasively acquire in vivo images of rodent models for PKD. The main goal of this work is to introduce the use of MRI as a tool for in vivo phenotypical characterization and drug monitoring in rodent models for PKD.

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KW - Total kidney volume (TKV)

KW - Ultra-high field (UHF) MRI

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