Use of t-butyldimethylsilylation in the gas chromatographic/mass spectrometric analysis of physiologic compounds found in plasma using electron-impact ionization

W. Frederick Schwenk, Peter J. Berg, Bernard Beaufrere, John M. Miles, Morey W. Haymond

Research output: Contribution to journalArticle

220 Citations (Scopus)

Abstract

The use of N-methyl-N-(t-butyldimethylsilyl)trifluoroacetamide to prepare the t-butyldimethylsilyl derivatives of a number of organic compounds (selected amino acids, α-keto acids, ketone bodies, free fatty acids, urea, glycerol, lactate, and pyruvate) is reported. These derivatives are particularly useful for gas chromatographic/mass spectrometric analysis involving the use of stable isotopes and selected ion monitoring, since a peak of sufficient abundance at 57 mass/charge units below the molecular ion was always present, and was the result of the loss of one t-butyl group. In each case, this fragment contained the entire skeleton of the original compound, which permitted easy analysis using electron-impact ionization of these compounds alone or when labeled with stable isotopes in any nonexchangeable position.

Original languageEnglish (US)
Pages (from-to)101-109
Number of pages9
JournalAnalytical Biochemistry
Volume141
Issue number1
DOIs
StatePublished - Aug 15 1984

Fingerprint

Impact ionization
Isotopes
Gases
Electrons
Ions
Derivatives
Keto Acids
Plasmas
Ketone Bodies
Pyruvic Acid
Organic compounds
Nonesterified Fatty Acids
Skeleton
Glycerol
Urea
Lactic Acid
Amino Acids
Monitoring
trifluoroacetamide

Keywords

  • amino acids
  • gas chromatography/mass spectrometry
  • glycerol
  • organic acids
  • t-butyldimethylsilyl derivatives
  • urea

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Use of t-butyldimethylsilylation in the gas chromatographic/mass spectrometric analysis of physiologic compounds found in plasma using electron-impact ionization. / Frederick Schwenk, W.; Berg, Peter J.; Beaufrere, Bernard; Miles, John M.; Haymond, Morey W.

In: Analytical Biochemistry, Vol. 141, No. 1, 15.08.1984, p. 101-109.

Research output: Contribution to journalArticle

Frederick Schwenk, W. ; Berg, Peter J. ; Beaufrere, Bernard ; Miles, John M. ; Haymond, Morey W. / Use of t-butyldimethylsilylation in the gas chromatographic/mass spectrometric analysis of physiologic compounds found in plasma using electron-impact ionization. In: Analytical Biochemistry. 1984 ; Vol. 141, No. 1. pp. 101-109.
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