TY - JOUR
T1 - Use of fluorescence indicators in receptor ligands
AU - Harikumar, Kaleeckal G.
AU - Miller, Laurence J.
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2015.
PY - 2015
Y1 - 2015
N2 - Fluorescence techniques can provide insights into the environment of fluorescence indicators incorporated within a ligand as it is bound to its receptor. Fluorescence indicators of different sizes and chemical characteristics can provide insights into the nature of the binding environment, the surrounding structures, and even into conformational changes associated with receptor activation. Methods for determining fluorescence spectral analysis, fluorescence quenching, fluorescence anisotropy, fluorescence lifetimes, and red edge excitation shifts of the ligand probes are described. The applications of these techniques to the CCK1 receptor occupied by alexa488-CCK and aladan-CCK, as examples of probes developed (1) by derivatization of an existing peptide and (2) by incorporation during peptide synthesis, are utilized as examples. These methods represent powerful tools to expand our understanding of the structure and molecular basis of ligand activation of G protein-coupled receptors.
AB - Fluorescence techniques can provide insights into the environment of fluorescence indicators incorporated within a ligand as it is bound to its receptor. Fluorescence indicators of different sizes and chemical characteristics can provide insights into the nature of the binding environment, the surrounding structures, and even into conformational changes associated with receptor activation. Methods for determining fluorescence spectral analysis, fluorescence quenching, fluorescence anisotropy, fluorescence lifetimes, and red edge excitation shifts of the ligand probes are described. The applications of these techniques to the CCK1 receptor occupied by alexa488-CCK and aladan-CCK, as examples of probes developed (1) by derivatization of an existing peptide and (2) by incorporation during peptide synthesis, are utilized as examples. These methods represent powerful tools to expand our understanding of the structure and molecular basis of ligand activation of G protein-coupled receptors.
KW - Cholecystokinin receptor
KW - Collisional quenching
KW - Emission spectra
KW - Excitation spectra
KW - Fluorescence anisotropy
KW - Fluorescence lifetime
KW - Fluorescent ligands
KW - Red edge excitation shift
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U2 - 10.1007/978-1-4939-2914-6_9
DO - 10.1007/978-1-4939-2914-6_9
M3 - Article
C2 - 26260598
AN - SCOPUS:84938887179
SN - 1064-3745
VL - 1335
SP - 115
EP - 130
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -