Use of flow cytometry in the diagnosis of lymphoproliferative disorders in fluid specimens

Gordon H. Yu, Norge Vergara, Erika M. Moore, Rebecca King

Research output: Contribution to journalReview article

13 Citations (Scopus)

Abstract

The diagnostic evaluation of fluid specimens, including serous effusions and cerebrospinal fluids (CSFs), can be challenging for a number of reasons. The evaluation of lymphoid proliferations in these specimens can be particularly problematic, given the frequent presence of coexisting inflammatory conditions and the manner in which these specimens are processed. As a result, immunophenotypic analysis of hematopoietic cell populations by flow cytometry has emerged as a useful ancillary study in the diagnosis of these specimens, both in patients with and without a previous history of a lymphoproliferative disorder. In this study, we review our experience with flow cytometry in fluid specimens over a four-year period. Flow cytometry was performed in 184 of 6,925 total cases (2.7% of all fluids). Flow cytometry was performed in 4.8% of pleural fluids (positive findings in 38%, negative in 40%, and atypical in 18%), 1.1% of peritoneal fluids (positive in 40%, negative in 50%, and atypical in 10%), 1.9% of pericardial fluids (positive in 67%, negative in 33%), and 1.9% of CSFs (positive in 23%, negative in 55%, atypical in 3%). The specimen submitted was inadequate for analysis in 9.2% of cases, most commonly with CSF specimens, but was not related to the volume of fluid submitted. Atypical flow cytometry findings and atypical morphologic findings in the context of negative flow cytometry results led to the definitive diagnosis of a lymphoproliferative disorder in a significant number of cases when repeat procedures and ancillary studies were performed.

Original languageEnglish (US)
Pages (from-to)664-670
Number of pages7
JournalDiagnostic cytopathology
Volume42
Issue number8
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

Lymphoproliferative Disorders
Flow Cytometry
Cerebrospinal Fluid
Ascitic Fluid
Population

Keywords

  • cerebrospinal fluid
  • effusion
  • flow cytometry
  • fluid cytology
  • lymphoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Cite this

Use of flow cytometry in the diagnosis of lymphoproliferative disorders in fluid specimens. / Yu, Gordon H.; Vergara, Norge; Moore, Erika M.; King, Rebecca.

In: Diagnostic cytopathology, Vol. 42, No. 8, 01.01.2014, p. 664-670.

Research output: Contribution to journalReview article

Yu, Gordon H. ; Vergara, Norge ; Moore, Erika M. ; King, Rebecca. / Use of flow cytometry in the diagnosis of lymphoproliferative disorders in fluid specimens. In: Diagnostic cytopathology. 2014 ; Vol. 42, No. 8. pp. 664-670.
@article{27e9ecce8d03463395f66ad67e99c283,
title = "Use of flow cytometry in the diagnosis of lymphoproliferative disorders in fluid specimens",
abstract = "The diagnostic evaluation of fluid specimens, including serous effusions and cerebrospinal fluids (CSFs), can be challenging for a number of reasons. The evaluation of lymphoid proliferations in these specimens can be particularly problematic, given the frequent presence of coexisting inflammatory conditions and the manner in which these specimens are processed. As a result, immunophenotypic analysis of hematopoietic cell populations by flow cytometry has emerged as a useful ancillary study in the diagnosis of these specimens, both in patients with and without a previous history of a lymphoproliferative disorder. In this study, we review our experience with flow cytometry in fluid specimens over a four-year period. Flow cytometry was performed in 184 of 6,925 total cases (2.7{\%} of all fluids). Flow cytometry was performed in 4.8{\%} of pleural fluids (positive findings in 38{\%}, negative in 40{\%}, and atypical in 18{\%}), 1.1{\%} of peritoneal fluids (positive in 40{\%}, negative in 50{\%}, and atypical in 10{\%}), 1.9{\%} of pericardial fluids (positive in 67{\%}, negative in 33{\%}), and 1.9{\%} of CSFs (positive in 23{\%}, negative in 55{\%}, atypical in 3{\%}). The specimen submitted was inadequate for analysis in 9.2{\%} of cases, most commonly with CSF specimens, but was not related to the volume of fluid submitted. Atypical flow cytometry findings and atypical morphologic findings in the context of negative flow cytometry results led to the definitive diagnosis of a lymphoproliferative disorder in a significant number of cases when repeat procedures and ancillary studies were performed.",
keywords = "cerebrospinal fluid, effusion, flow cytometry, fluid cytology, lymphoma",
author = "Yu, {Gordon H.} and Norge Vergara and Moore, {Erika M.} and Rebecca King",
year = "2014",
month = "1",
day = "1",
doi = "10.1002/dc.23106",
language = "English (US)",
volume = "42",
pages = "664--670",
journal = "Diagnostic Cytopathology",
issn = "8755-1039",
publisher = "Wiley-Liss Inc.",
number = "8",

}

TY - JOUR

T1 - Use of flow cytometry in the diagnosis of lymphoproliferative disorders in fluid specimens

AU - Yu, Gordon H.

AU - Vergara, Norge

AU - Moore, Erika M.

AU - King, Rebecca

PY - 2014/1/1

Y1 - 2014/1/1

N2 - The diagnostic evaluation of fluid specimens, including serous effusions and cerebrospinal fluids (CSFs), can be challenging for a number of reasons. The evaluation of lymphoid proliferations in these specimens can be particularly problematic, given the frequent presence of coexisting inflammatory conditions and the manner in which these specimens are processed. As a result, immunophenotypic analysis of hematopoietic cell populations by flow cytometry has emerged as a useful ancillary study in the diagnosis of these specimens, both in patients with and without a previous history of a lymphoproliferative disorder. In this study, we review our experience with flow cytometry in fluid specimens over a four-year period. Flow cytometry was performed in 184 of 6,925 total cases (2.7% of all fluids). Flow cytometry was performed in 4.8% of pleural fluids (positive findings in 38%, negative in 40%, and atypical in 18%), 1.1% of peritoneal fluids (positive in 40%, negative in 50%, and atypical in 10%), 1.9% of pericardial fluids (positive in 67%, negative in 33%), and 1.9% of CSFs (positive in 23%, negative in 55%, atypical in 3%). The specimen submitted was inadequate for analysis in 9.2% of cases, most commonly with CSF specimens, but was not related to the volume of fluid submitted. Atypical flow cytometry findings and atypical morphologic findings in the context of negative flow cytometry results led to the definitive diagnosis of a lymphoproliferative disorder in a significant number of cases when repeat procedures and ancillary studies were performed.

AB - The diagnostic evaluation of fluid specimens, including serous effusions and cerebrospinal fluids (CSFs), can be challenging for a number of reasons. The evaluation of lymphoid proliferations in these specimens can be particularly problematic, given the frequent presence of coexisting inflammatory conditions and the manner in which these specimens are processed. As a result, immunophenotypic analysis of hematopoietic cell populations by flow cytometry has emerged as a useful ancillary study in the diagnosis of these specimens, both in patients with and without a previous history of a lymphoproliferative disorder. In this study, we review our experience with flow cytometry in fluid specimens over a four-year period. Flow cytometry was performed in 184 of 6,925 total cases (2.7% of all fluids). Flow cytometry was performed in 4.8% of pleural fluids (positive findings in 38%, negative in 40%, and atypical in 18%), 1.1% of peritoneal fluids (positive in 40%, negative in 50%, and atypical in 10%), 1.9% of pericardial fluids (positive in 67%, negative in 33%), and 1.9% of CSFs (positive in 23%, negative in 55%, atypical in 3%). The specimen submitted was inadequate for analysis in 9.2% of cases, most commonly with CSF specimens, but was not related to the volume of fluid submitted. Atypical flow cytometry findings and atypical morphologic findings in the context of negative flow cytometry results led to the definitive diagnosis of a lymphoproliferative disorder in a significant number of cases when repeat procedures and ancillary studies were performed.

KW - cerebrospinal fluid

KW - effusion

KW - flow cytometry

KW - fluid cytology

KW - lymphoma

UR - http://www.scopus.com/inward/record.url?scp=84904579904&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904579904&partnerID=8YFLogxK

U2 - 10.1002/dc.23106

DO - 10.1002/dc.23106

M3 - Review article

C2 - 24554583

AN - SCOPUS:84904579904

VL - 42

SP - 664

EP - 670

JO - Diagnostic Cytopathology

JF - Diagnostic Cytopathology

SN - 8755-1039

IS - 8

ER -