Measurement of individual muscle tension in a clinical setting has yet to be achieved. Previous investigators have suggested that the tension in skeletal muscle, comprised of approximately 70% fluid, could be determined using interstitial muscle fluid pressure (IMP). A computational model is needed to aid in understanding IMP distribution in muscles of varying geometry and contractile states without exhaustive testing. The first aim of this study was to determine a set of transversely isotropic material properties (i.e., permeability, relaxed modulus, and drained Poisson's ratio) for excised skeletal muscle using inverse finite element analysis with a poroelastic constitutive formulation on tension data from either longitudinal or transverse uniaxial load-relaxation tests of skeletal muscle tissue. The second aim was to compare pore pressure estimated from a model to experimental pressure measurements to assess its ability to accurately predict IMP. Results of this study indicated that skeletal muscle was transversely isotropic under load-relaxation as demonstrated by significant differences in the drained Poisson's ratio. It was also noted that the drained Poisson's ratios under both longitudinal and transverse loading were negative in these tests of excised muscle tissue. Pore pressure calculated with this model provided a good prediction of the development of IMP. These results point to the benefit of using a poroelastic model of skeletal muscle to predict IMP.