Use of a, hollow fiber bioreactor to collect extracellular vesicles from cells in culture

Irene K. Yan, Neha Shukla, David A. Borrelli, Tushar C Patel

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

Current approaches for collection of extracellular vesicles (EV) are based on classical cell culture media production. This involves collection from cells grown in flasks, and can require multiple rounds of centrifugation or filtration, followed by ultracentrifugation or density gradient centrifugation. There are several limitations of these approaches, for example, they require a large input volume, the yield and concentration is low, and the process is time consuming. Most cell cultures require the use of fetal bovine serum which contains a large amount of endogenous EV that can contaminate isolations of cell-derived EVs. The use of cell cultures within a hollow fiber bioreactor could address many of these limitations and produce a continuous source of highly concentrated EVs without contamination from serum EVs, and that are suitable for downstream applications.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages35-41
Number of pages7
Volume1740
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1740
ISSN (Print)1064-3745

Keywords

  • Exosomes
  • Extracellular vesicles
  • Hollow fiber bioreactor
  • Isolation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Yan, I. K., Shukla, N., Borrelli, D. A., & Patel, T. C. (2018). Use of a, hollow fiber bioreactor to collect extracellular vesicles from cells in culture. In Methods in Molecular Biology (Vol. 1740, pp. 35-41). (Methods in Molecular Biology; Vol. 1740). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7652-2_4