Use of a, hollow fiber bioreactor to collect extracellular vesicles from cells in culture

Irene K. Yan, Neha Shukla, David A. Borrelli, Tushar C Patel

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Current approaches for collection of extracellular vesicles (EV) are based on classical cell culture media production. This involves collection from cells grown in flasks, and can require multiple rounds of centrifugation or filtration, followed by ultracentrifugation or density gradient centrifugation. There are several limitations of these approaches, for example, they require a large input volume, the yield and concentration is low, and the process is time consuming. Most cell cultures require the use of fetal bovine serum which contains a large amount of endogenous EV that can contaminate isolations of cell-derived EVs. The use of cell cultures within a hollow fiber bioreactor could address many of these limitations and produce a continuous source of highly concentrated EVs without contamination from serum EVs, and that are suitable for downstream applications.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages35-41
Number of pages7
Volume1740
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1740
ISSN (Print)1064-3745

Fingerprint

Bioreactors
Cell Culture Techniques
Density Gradient Centrifugation
Cell Separation
Ultracentrifugation
Serum
Centrifugation
Culture Media
Extracellular Vesicles

Keywords

  • Exosomes
  • Extracellular vesicles
  • Hollow fiber bioreactor
  • Isolation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yan, I. K., Shukla, N., Borrelli, D. A., & Patel, T. C. (2018). Use of a, hollow fiber bioreactor to collect extracellular vesicles from cells in culture. In Methods in Molecular Biology (Vol. 1740, pp. 35-41). (Methods in Molecular Biology; Vol. 1740). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7652-2_4

Use of a, hollow fiber bioreactor to collect extracellular vesicles from cells in culture. / Yan, Irene K.; Shukla, Neha; Borrelli, David A.; Patel, Tushar C.

Methods in Molecular Biology. Vol. 1740 Humana Press Inc., 2018. p. 35-41 (Methods in Molecular Biology; Vol. 1740).

Research output: Chapter in Book/Report/Conference proceedingChapter

Yan, IK, Shukla, N, Borrelli, DA & Patel, TC 2018, Use of a, hollow fiber bioreactor to collect extracellular vesicles from cells in culture. in Methods in Molecular Biology. vol. 1740, Methods in Molecular Biology, vol. 1740, Humana Press Inc., pp. 35-41. https://doi.org/10.1007/978-1-4939-7652-2_4
Yan IK, Shukla N, Borrelli DA, Patel TC. Use of a, hollow fiber bioreactor to collect extracellular vesicles from cells in culture. In Methods in Molecular Biology. Vol. 1740. Humana Press Inc. 2018. p. 35-41. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7652-2_4
Yan, Irene K. ; Shukla, Neha ; Borrelli, David A. ; Patel, Tushar C. / Use of a, hollow fiber bioreactor to collect extracellular vesicles from cells in culture. Methods in Molecular Biology. Vol. 1740 Humana Press Inc., 2018. pp. 35-41 (Methods in Molecular Biology).
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