Use of 2-D DIGE analysis reveals altered phosphorylation in a tropomyosin mutant (Glu54Lys) linked to dilated cardiomyopathy

Chad M. Warren, Grace M. Arteaga, Sudarsan Rajan, Rafeeq P.H. Ahmed, David F. Wieczorek, R. John Solaro

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Current electrophoretic methods have not been optimized to fully separate post-translationally modified mutant forms of tropomyosin (Tm) from wild-type cardiac samples. We describe here a method employing a modified 2-D PAGE/2-D DIGE protocol, to fully separate native, mutant (E54K), and phosphorylated forms of Tm. Our data demonstrate the first evidence of a significant (∼40%) decrease in Tm phosphorylation in transgenic compared to non-transgenic mouse hearts, and indicate that altered phosphorylation may be a significant factor in the linkage of the E54K mutation to dilated cardiomyopathy.

Original languageEnglish (US)
Pages (from-to)100-105
Number of pages6
JournalProteomics
Volume8
Issue number1
DOIs
StatePublished - Jan 2008

Keywords

  • 2-D DIGE
  • Familial cardiomyopathies
  • Kinases
  • Phosphatases
  • Sarcomere

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Use of 2-D DIGE analysis reveals altered phosphorylation in a tropomyosin mutant (Glu54Lys) linked to dilated cardiomyopathy'. Together they form a unique fingerprint.

  • Cite this

    Warren, C. M., Arteaga, G. M., Rajan, S., Ahmed, R. P. H., Wieczorek, D. F., & Solaro, R. J. (2008). Use of 2-D DIGE analysis reveals altered phosphorylation in a tropomyosin mutant (Glu54Lys) linked to dilated cardiomyopathy. Proteomics, 8(1), 100-105. https://doi.org/10.1002/pmic.200700772