Urocortinergic neurons respond in a differentiated manner to various acute stressors in the Edinger-Westphal nucleus in the rat

Balázs Gaszner, Valér Csernus, Tamas Kozicz

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Corticotropin-releasing factor (CRF) was implicated as being a major contributor to the neurochemically mediated central regulation of stress response; however, an increasing body of evidence suggests that, besides CRF, other members of this neuropeptide family, such as urocortin (Ucn), may also play a role in modifying the efferent components of immune, endocrine, and behavioral responses to stress. Ucn's distribution in the rat brain has been demonstrated, with the most abundant Ucn-immunoreactive perikarya present in the Edinger-Westphal nucleus (E-WN). Acute pain and immobilization stresses recruit E-WN neurons, however, the activation pattern of E-WN Ucn neurons in response to various acute systemic and neurogenic challenges has not been compared in a single study. We therefore combined quantitative Fos imaging as a marker for neuronal activation with urocortin immunohistochemistry to visualize neurons induced by intravenous lipopolysaccharide (LPS; 100 μg/kg), ether inhalation, restraint, hyperosmotic (1.5 M NaCl i.p.), and hypotensive hemorrhage challenges. Neurons in the E-WN responded with the strongest Fos induction to LPS, but ether and restraint stress also resulted in massive Fos immunoreactivity 2 hours after stress. Unexpectedly, hyperosmotic and hypotensive hemorrhage stresses did not induce urocortinergic perikarya in this brain area 2 hours poststress. This challenge-specific recruitment of E-WN neurons was independent of stress-induced adrenal response. The biological significance and the stress-specific activation of E-WN urocortinergic neurons will be discussed.

Original languageEnglish (US)
Pages (from-to)170-179
Number of pages10
JournalJournal of Comparative Neurology
Volume480
Issue number2
DOIs
StatePublished - Dec 6 2004
Externally publishedYes

Fingerprint

Urocortins
Neurons
Corticotropin-Releasing Hormone
Ether
Hemorrhage
Brain
Acute Pain
Neuropeptides
Immobilization
Inhalation
Lipopolysaccharides
Edinger-Westphal Nucleus
Immunohistochemistry

Keywords

  • Immediate early genes
  • Immunohistochemistry
  • Neurogenic stress
  • Systemic stress
  • Urocortin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Urocortinergic neurons respond in a differentiated manner to various acute stressors in the Edinger-Westphal nucleus in the rat. / Gaszner, Balázs; Csernus, Valér; Kozicz, Tamas.

In: Journal of Comparative Neurology, Vol. 480, No. 2, 06.12.2004, p. 170-179.

Research output: Contribution to journalArticle

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