Urocortin expression in the Edinger-Westphal nucleus is down-regulated in transgenic mice over-expressing neuronal corticotropin-releasing factor

Tamas Kozicz, A. Korosi, C. Korsman, D. Tilburg-Ouwens, L. Groenink, J. Veening, J. Van Der Gugten, E. Roubos, B. Olivier

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

In recent years a large body of evidence has emerged linking chronic stress with increased vulnerability for depression and anxiety disorders. As corticotropin-releasing factor (CRF) is hypersecreted under these psychological conditions, we used our CRF-overexpressing (CRF-OE) mouse line to study underlying brain mechanisms possibly causing these disorders. Urocortin (Ucn), a recently discovered member of the CRF peptide family may play a role in the pathophysiology of stress-induced disorders. Stressors recruit Ucn-immunoreactive neurons in the Edinger-Westphal nucleus (E-WN), which is the major site of Ucn expression. Furthermore, E-WN Ucn mRNA levels are upregulated in CRF-deficient mice. Based on these findings, we hypothesized the down-regulation of E-WN Ucn in CRF-OE mice and consequently, altered responsiveness to stressful stimuli. Our results support this hypothesis as we found weaker immunohistochemical labeling with anti-Ucn and a six times weaker Ucn mRNA signal in E-WN in CRF-OE mice. Moreover, E-WN Ucn-expressing neurons mounted a response to acute challenge in CRF-OE mice too. From these results it is concluded that the CRF and E-WN Ucn neuronal systems work in concert in response to acute challenges, but are inversely regulated in their activities during chronic hyperactivity of the hypothalamo-pituitary-adrenal axis.

Original languageEnglish (US)
Pages (from-to)589-594
Number of pages6
JournalNeuroscience
Volume123
Issue number3
DOIs
StatePublished - Jan 1 2004
Externally publishedYes

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Urocortins
Corticotropin-Releasing Hormone
Transgenic Mice
Edinger-Westphal Nucleus
Neurons
Messenger RNA
Anxiety Disorders
Down-Regulation
Depression
Psychology

Keywords

  • Acute ether stress
  • In situ hybridization
  • Quantitative Fos immunohistochemistry

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Urocortin expression in the Edinger-Westphal nucleus is down-regulated in transgenic mice over-expressing neuronal corticotropin-releasing factor. / Kozicz, Tamas; Korosi, A.; Korsman, C.; Tilburg-Ouwens, D.; Groenink, L.; Veening, J.; Van Der Gugten, J.; Roubos, E.; Olivier, B.

In: Neuroscience, Vol. 123, No. 3, 01.01.2004, p. 589-594.

Research output: Contribution to journalArticle

Kozicz, T, Korosi, A, Korsman, C, Tilburg-Ouwens, D, Groenink, L, Veening, J, Van Der Gugten, J, Roubos, E & Olivier, B 2004, 'Urocortin expression in the Edinger-Westphal nucleus is down-regulated in transgenic mice over-expressing neuronal corticotropin-releasing factor', Neuroscience, vol. 123, no. 3, pp. 589-594. https://doi.org/10.1016/j.neuroscience.2003.10.042
Kozicz, Tamas ; Korosi, A. ; Korsman, C. ; Tilburg-Ouwens, D. ; Groenink, L. ; Veening, J. ; Van Der Gugten, J. ; Roubos, E. ; Olivier, B. / Urocortin expression in the Edinger-Westphal nucleus is down-regulated in transgenic mice over-expressing neuronal corticotropin-releasing factor. In: Neuroscience. 2004 ; Vol. 123, No. 3. pp. 589-594.
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