TY - JOUR
T1 - Uridine transport in human erythrocytes
T2 - data from normal subjects and from patients with renal failure
AU - Fervenza, FC
AU - Meredith, D.
AU - Ellory, JC
AU - Hendry, BM
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1991/1/1
Y1 - 1991/1/1
N2 - Erythrocyte uridine transport has been studied in eight normal individuals and eight patients on haemodialysis for chronic renal failure. The initial rate of zero‐trans uridine influx at 37 degrees C has been measured as a function of extracellular uridine concentration using [14C]‐labelled uridine. The results are consistent with Michaelis‐Menten kinetics. In normal humans the mean Vmax for uridine influx was 32.8 +/‐ 6.4 mumol (1 cells)‐1 s‐1 (mean +/‐ S.D.) and the mean Km was 190 +/‐ 12.3 microM. The measurements made in renal failure patients were not significantly different (mean Vmax 30.1 +/‐ 7.1 mumol (1 cells)‐1 s‐1, mean Km, 185 +/‐ 13.2 microM). These results are discussed with reference to the reported data on uridine transport in human erythrocytes at temperatures between 4 and 35 degrees C; it is suggested that zero‐trans uridine influx shows a decrease in temperature dependence above 25 degrees C. The Vmax for zero‐trans uridine influx at 37 degrees C in normal erythrocytes represents a turnover number for the nucleoside transporter of 180 uridine molecules per second.
AB - Erythrocyte uridine transport has been studied in eight normal individuals and eight patients on haemodialysis for chronic renal failure. The initial rate of zero‐trans uridine influx at 37 degrees C has been measured as a function of extracellular uridine concentration using [14C]‐labelled uridine. The results are consistent with Michaelis‐Menten kinetics. In normal humans the mean Vmax for uridine influx was 32.8 +/‐ 6.4 mumol (1 cells)‐1 s‐1 (mean +/‐ S.D.) and the mean Km was 190 +/‐ 12.3 microM. The measurements made in renal failure patients were not significantly different (mean Vmax 30.1 +/‐ 7.1 mumol (1 cells)‐1 s‐1, mean Km, 185 +/‐ 13.2 microM). These results are discussed with reference to the reported data on uridine transport in human erythrocytes at temperatures between 4 and 35 degrees C; it is suggested that zero‐trans uridine influx shows a decrease in temperature dependence above 25 degrees C. The Vmax for zero‐trans uridine influx at 37 degrees C in normal erythrocytes represents a turnover number for the nucleoside transporter of 180 uridine molecules per second.
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U2 - 10.1113/expphysiol.1991.sp003480
DO - 10.1113/expphysiol.1991.sp003480
M3 - Article
C2 - 2015074
AN - SCOPUS:0026025841
SN - 0958-0670
VL - 76
SP - 53
EP - 58
JO - Experimental physiology
JF - Experimental physiology
IS - 1
ER -