Ureaplasma parvum prosthetic joint infection detected by PCR

John J. Farrell, Joshua A. Larson, Jeffrey W. Akeson, Kristin S. Lowery, Megan A. Rounds, Rangarajan Sampath, Robert A. Bonomo, Robin Patel

Research output: Contribution to journalArticle

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Abstract

We describe the first reported case of Ureaplasma parvum prosthetic joint infection (PJI) detected by PCR. Ureaplasma species do not possess a cell wall and are usually associated with colonization and infection of mucosal surfaces (not prosthetic material). U. parvum is a relatively new species name for certain serovars of Ureaplasma urealyticum, and PCR is useful for species determination. Our patient presented with late infection of his right total knee arthroplasty. Intraoperative fluid and tissue cultures and pre- and postoperative synovial fluid cultures were all negative. To discern the pathogen, we employed PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS). Our patient's failure to respond to empirical antimicrobial treatment and our previous experience with PCR/ESI-MS in culture-negative cases of infection prompted us to use this approach over other diagnostic modalities. PCR/ESI-MS detected U. parvum in all samples. U. parvum-specific PCR testing was performed on all synovial fluid samples to confirm the U. parvum detection.

Original languageEnglish (US)
Pages (from-to)2248-2250
Number of pages3
JournalJournal of Clinical Microbiology
Volume52
Issue number6
DOIs
StatePublished - 2014

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Ureaplasma
Joints
Polymerase Chain Reaction
Electrospray Ionization Mass Spectrometry
Infection
Synovial Fluid
Ureaplasma urealyticum
Knee Replacement Arthroplasties
Cell Wall
Names

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Farrell, J. J., Larson, J. A., Akeson, J. W., Lowery, K. S., Rounds, M. A., Sampath, R., ... Patel, R. (2014). Ureaplasma parvum prosthetic joint infection detected by PCR. Journal of Clinical Microbiology, 52(6), 2248-2250. https://doi.org/10.1128/JCM.00432-14

Ureaplasma parvum prosthetic joint infection detected by PCR. / Farrell, John J.; Larson, Joshua A.; Akeson, Jeffrey W.; Lowery, Kristin S.; Rounds, Megan A.; Sampath, Rangarajan; Bonomo, Robert A.; Patel, Robin.

In: Journal of Clinical Microbiology, Vol. 52, No. 6, 2014, p. 2248-2250.

Research output: Contribution to journalArticle

Farrell, JJ, Larson, JA, Akeson, JW, Lowery, KS, Rounds, MA, Sampath, R, Bonomo, RA & Patel, R 2014, 'Ureaplasma parvum prosthetic joint infection detected by PCR', Journal of Clinical Microbiology, vol. 52, no. 6, pp. 2248-2250. https://doi.org/10.1128/JCM.00432-14
Farrell JJ, Larson JA, Akeson JW, Lowery KS, Rounds MA, Sampath R et al. Ureaplasma parvum prosthetic joint infection detected by PCR. Journal of Clinical Microbiology. 2014;52(6):2248-2250. https://doi.org/10.1128/JCM.00432-14
Farrell, John J. ; Larson, Joshua A. ; Akeson, Jeffrey W. ; Lowery, Kristin S. ; Rounds, Megan A. ; Sampath, Rangarajan ; Bonomo, Robert A. ; Patel, Robin. / Ureaplasma parvum prosthetic joint infection detected by PCR. In: Journal of Clinical Microbiology. 2014 ; Vol. 52, No. 6. pp. 2248-2250.
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