Ureaplasma parvum causes hyperammonemia in a pharmacologically immunocompromised murine model

X. Wang, K. E. Greenwood-Quaintance, M. J. Karau, D. R. Block, Jayawant Mandrekar, S. A. Cunningham, J. M. Mallea, Robin Patel

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Abstract

A relationship between hyperammonemia and Ureaplasma infection has been shown in lung transplant recipients. We have demonstrated that Ureaplasma urealyticum causes hyperammonemia in a novel immunocompromised murine model. Herein, we determined whether Ureaplasma parvum can do the same. Male C3H mice were given mycophenolate mofetil, tacrolimus, and prednisone for 7 days, and then challenged with U. parvum intratracheally (IT) and/or intraperitoneally (IP), while continuing immunosuppression over 6 days. Plasma ammonia concentrations were determined and compared using Wilcoxon rank-sum tests. Plasma ammonia concentrations of immunosuppressed mice challenged IT/IP with spent broth (median, 188 μmol/L; range, 102–340 μmol/L) were similar to those of normal (median, 226 μmol/L; range, 154–284 μmol/L, p > 0.05), uninfected immunosuppressed (median, 231 μmol/L; range, 122–340 μmol/L, p > 0.05), and U. parvum IT/IP challenged immunocompetent (median, 226 μmol/L; range, 130–330 μmol/L, p > 0.05) mice. Immunosuppressed mice challenged with U. parvum IT/IP (median 343 μmol/L; range 136–1,000 μmol/L) or IP (median 307 μmol/L; range 132–692 μmol/L) had higher plasma ammonia concentrations than those challenged IT/IP with spent broth (p < 0.001). U. parvum can cause hyperammonemia in pharmacologically immunocompromised mice.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
DOIs
StateAccepted/In press - Oct 28 2016

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ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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