Upstream stimulatory factors regulate the C/EBPα gene during differentiation of 3T3-L1 preadipocytes

Jae woo Kim, Henrik Monila, Akhilesh Pandey, M. Daniel Lane

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

During adipocyte differentiation, CCAAT/enhancer-binding protein α (C/EBPα) functions as a pleiotropic transcriptional activator of numerous adipocyte genes. The promoter of the C/EBPα gene has an E-box upstream of C/EBP binding site. Deletion or mutation of the E-box decreases promoter activity, suggesting that the E-box participates in the regulation of C/EBPα expression. Protein binding to the E-box during the adipocyte differentiation is increased as indicated by EMSA and UV cross-linking. Purification of the E-box binding proteins from differentiated 3T3-L1 adipocytes, showed that USF and AP-4 are associated with the E-box. Supershift analysis showed that USF1 and USF2 bind to this element as heterodimers, whereas the addition of anti-AP-4 antibody enhanced the binding complex, suggesting that AP-4 negatively regulates the promoter activity. The expression of AP-4 is reciprocally regulated with USF-1 during adipocyte differentiation. These findings suggest that USF-1 and 2 play roles in C/EBPα expression, whereas the AP-4 represses it.

Original languageEnglish (US)
Pages (from-to)517-521
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume354
Issue number2
DOIs
StatePublished - Mar 9 2007

Keywords

  • AP-4
  • Adipocyte differentiation
  • C/EBPα
  • DNA binding activity
  • E-box
  • Promoter
  • USF

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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