Upregulation of secretin receptors on cholangiocytes after bile duct ligation

P. S. Tietz, E. M. Hadac, Laurence J Miller, Nicholas F La Russo

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Secretin not only increases ductular bile secretion in vivo in rats after bile duct ligation (BDL) [1], but also increases cAMP levels and stimulates exocytosis in isolated cholangiocytes [2]. Although we have previously reported that secretin receptor mRNA was upregulated in cholangiocytes after BDL [3], the cholangiocyte secretin receptor has not been functionally characterized or quantified after BDL. In this work, we used a novel, photolabile and biologically active analogue of secretin to quantify and characterize secretin receptors on cholangiocytes isolated from normal and BDL rats. The cholangiocyte secretin receptor bound radioligand with high affinity and in a rapid, reversible, and temperature-dependent manner. While receptors on cholangiocytes from normal and BDL rats were functionally and biochemically identical, receptor density on cholangiocytes was increased 5-fold following BDL. The combination of increased cell number with increased functional secretin receptors per cell is due to the fact that cholangiocyte hyperplasia represents a reactive response to a cholestatic condition and this effort on the part of the organism to maintain bile secretion, explains the increased hormone-responsive choleresis observed after BDL and may reflect an adaptive response of the organism to cholestasis.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalRegulatory Peptides
Volume97
Issue number1
DOIs
StatePublished - Feb 2 2001

Fingerprint

Bile Ducts
Ducts
Ligation
Up-Regulation
Rats
Secretin
Bile
Cholestasis
Exocytosis
secretin receptor
Hyperplasia
Cell Count
Hormones
Messenger RNA
Temperature

Keywords

  • Pancreatic
  • Photoaffinity labeling
  • Receptor
  • Secretin

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Upregulation of secretin receptors on cholangiocytes after bile duct ligation. / Tietz, P. S.; Hadac, E. M.; Miller, Laurence J; La Russo, Nicholas F.

In: Regulatory Peptides, Vol. 97, No. 1, 02.02.2001, p. 1-6.

Research output: Contribution to journalArticle

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