Upregulation of dynamin II expression during the acquisition of a mature pancreatic acinar cell phenotype

Tiffany A. Cook, Kristin J. Mesa, Brian A. Gebelein, Raul A. Urrutia

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Members of the dynamin superfamily are GTPases which have been shown to support receptor-mediated endocytosis in vivo and bind to growth factor receptor-associated proteins in vitro. In acinar cells of the pancreas, receptor-mediated endocytosis is very important for the recycling of membranes after secretory granule release. Therefore, characterization of the molecular machinery responsible for this process is critical for a better understanding of this phenomenon. In this study we sought to determine the expression pattern of the endocytic GTPase dynamin II during pancreatic acinar cell differentiation in developing rat embryos and in dexamethasone-treated AR42J cells using Western blot, Northern blot, and immunocytochemical analyses. During pancreatic development, dynamin immunoreactivity is almost (indetectable until day E17 but undergoes significant upregulation in acinar cells starting at E18. In addition, the levels of dynamin mRNA and protein in AR42J cells increase approximately threefold during dexamethasone-induced acinat differentiation. The increase in dynamin levels that occurs in both embryonic pancreatic cells and dexamethasone-treated AR42J cells correlates with the establishment of a more differentiated acinar phenotype. Therefore, these results suggest a potential role for dynamin in supporting receptor-mediated endocytosis in mature pancreatic acinar cells.

Original languageEnglish (US)
Pages (from-to)1373-1378
Number of pages6
JournalPediatrics
Volume97
Issue number3
StatePublished - 1996

Fingerprint

Dynamin II
Dynamins
Acinar Cells
Up-Regulation
Phenotype
Endocytosis
Dexamethasone
GTP Phosphohydrolases
Growth Factor Receptors
Secretory Vesicles
Northern Blotting
Cell Differentiation
Pancreas
Proteins
Embryonic Structures
Western Blotting
Messenger RNA
Membranes

Keywords

  • Acinar
  • AR42J
  • Dynamin
  • Pancreatic cell differetniation

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Cook, T. A., Mesa, K. J., Gebelein, B. A., & Urrutia, R. A. (1996). Upregulation of dynamin II expression during the acquisition of a mature pancreatic acinar cell phenotype. Pediatrics, 97(3), 1373-1378.

Upregulation of dynamin II expression during the acquisition of a mature pancreatic acinar cell phenotype. / Cook, Tiffany A.; Mesa, Kristin J.; Gebelein, Brian A.; Urrutia, Raul A.

In: Pediatrics, Vol. 97, No. 3, 1996, p. 1373-1378.

Research output: Contribution to journalArticle

Cook, TA, Mesa, KJ, Gebelein, BA & Urrutia, RA 1996, 'Upregulation of dynamin II expression during the acquisition of a mature pancreatic acinar cell phenotype', Pediatrics, vol. 97, no. 3, pp. 1373-1378.
Cook, Tiffany A. ; Mesa, Kristin J. ; Gebelein, Brian A. ; Urrutia, Raul A. / Upregulation of dynamin II expression during the acquisition of a mature pancreatic acinar cell phenotype. In: Pediatrics. 1996 ; Vol. 97, No. 3. pp. 1373-1378.
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