Upon infection, cellular WD repeat-containing protein 5 (WDR5) localizes to cytoplasmic inclusion bodies and enhances measles virus replication

Dzwokai Ma, Cyril X. George, Jason L. Nomburg, Christian K. Pfaller, Roberto Cattaneo, Charles E. Samuel

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Replication of negative-strand RNA viruses occurs in association with discrete cytoplasmic foci called inclusion bodies. Whereas inclusion bodies represent a prominent subcellular structure induced by viral infection, our knowledge of the cellular protein components involved in inclusion body formation and function is limited. Using measles virus-infected HeLa cells, we found that the WD repeatcontaining protein 5 (WDR5), a subunit of histone H3 lysine 4 methyltransferases, was selectively recruited to virus-induced inclusion bodies. Furthermore, WDR5 was found in complexes containing viral proteins associated with RNA replication. WDR5 was not detected with mitochondria, stress granules, or other known secretory or endocytic compartments of infected cells. WDR5 deficiency decreased both viral protein production and infectious virus yields. Interferon production was modestly increased in WDR5-deficient cells. Thus, our study identifies WDR5 as a novel viral inclusion body-associated cellular protein and suggests a role for WDR5 in promoting viral replication.

Original languageEnglish (US)
Article numbere01726-17
JournalJournal of Virology
Volume92
Issue number5
DOIs
StatePublished - Mar 1 2018

Fingerprint

Measles virus
cytoplasmic inclusions
Inclusion Bodies
inclusion bodies
Virus Replication
virus replication
Infection
infection
Proteins
proteins
Viral Proteins
viral proteins
Viral Inclusion Bodies
Viruses
Protein Deficiency
WD40 Repeats
RNA Viruses
viruses
Methyltransferases
Virus Diseases

Keywords

  • Inclusion body
  • Innate immunity
  • Measles virus
  • WD repeat-containing protein
  • WDR5

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Upon infection, cellular WD repeat-containing protein 5 (WDR5) localizes to cytoplasmic inclusion bodies and enhances measles virus replication. / Ma, Dzwokai; George, Cyril X.; Nomburg, Jason L.; Pfaller, Christian K.; Cattaneo, Roberto; Samuel, Charles E.

In: Journal of Virology, Vol. 92, No. 5, e01726-17, 01.03.2018.

Research output: Contribution to journalArticle

Ma, Dzwokai ; George, Cyril X. ; Nomburg, Jason L. ; Pfaller, Christian K. ; Cattaneo, Roberto ; Samuel, Charles E. / Upon infection, cellular WD repeat-containing protein 5 (WDR5) localizes to cytoplasmic inclusion bodies and enhances measles virus replication. In: Journal of Virology. 2018 ; Vol. 92, No. 5.
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