Abstract
We determined the existence and role of β2-adrenergic receptor in cultured BAECs through the effect of a β-blocker having NO releasing action; 3, 4-dihydro-8(2-hydroxy-3-isopropylamino)-propoxy-3-nitroxy-2H-1-benzopyran; nipradilol on eNOS and eNOS regulatory protein caveolin-1. β2receptor exists in BAECs. eNOS mRNA and protein were up-regulated by its treatment whereas those of caveolin were not altered considerably. This eNOS up-regulatory action was abolished by β2receptor antagonist, ICI-118551. Increase of NO metabolites, protein and mRNA of eNOS was also partially inhibited by co-treatment of NOS inhibitor, L-NA with nipradilol. This is the first investigation of the action of non-selective β blocker on eNOS through β2receptor. The drug increases NO on incubation with BAECs about 50% as a NO donor and about 50% as results of eNOS up-regulation.
Original language | English (US) |
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Pages (from-to) | 589-594 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 280 |
Issue number | 3 |
DOIs | |
State | Published - Jan 26 2001 |
Keywords
- Caveo-lin-1
- Endothelial nitric oxide synthase
- Nitric oxide
- β-adrenergic receptor antagonist
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology