TY - JOUR
T1 - Unique astrocyte ribbon in adult human brain contains neural stem cells but lacks chain migration
AU - Sanai, Hader
AU - Tramontin, Anthony D.
AU - Quiñones-Hinojosa, Alfredo
AU - Barbaro, Nicholas M.
AU - Gupta, Halin
AU - Kunwar, Sandeep
AU - Lawton, Michael T.
AU - McDermott, Michael W.
AU - Parsa, Andrew T.
AU - Verdugo, José Manuel Garcia
AU - Berger, Mitchel S.
AU - Alvarez-Buylla, Arturo
N1 - Funding Information:
Acknowledgements We thank G. E. Vates and J. W. Chiong for comments on the manuscript; A. Bollen, D. Gaskin, T. Tihan and S. R. VandenBerg for assistance with pathology material; A. Leong for stealth neuronavigational imagery; and Z. Mirzadeh for imaging. This work was supported by a gift from Frances and John Bowes, by the William Siebrandt Glioblastoma Fund and grants from the NIH. A.A-B. is the Heather and Melanie Muss Professor. N.S. is supported by the HHMI and an American Brain Tumor Association David B. Anderson Fellowship. A.D.T. is supported by the Damon Runyon Cancer Research Foundation.
Funding Information:
Acknowledgements We thank G. Jacobson and H. Kornberg, J. Paulsson, M. Savageau and A. Sengupta for discussions and suggestions; H. Bujard and R. Lutz for supplying the pZ vector system; and D. Boyd for help with the l-InCh technique. We thank D. Raut for his assistance with the initial lactose measurements and the construction of plasmids and strains. We also thank A. Becskei and J. Pedraza for critically reviewing the manuscript. This work was supported by NIH and DARPA grants, and an NSF-CAREER grant.
PY - 2004/2/19
Y1 - 2004/2/19
N2 - The subventricular zone (SVZ) is a principal source of adult neural stem cells in the rodent brain, generating thousands of olfactory bulb neurons every day. If the adult human brain contains a comparable germinal region, this could have considerable implications for future neuroregenerative therapy. Stem cells have been isolated from the human brain, but the identity, organization and function of adult neural stem cells in the human SVZ are unknown. Here we describe a ribbon of SVZ astrocytes lining the lateral ventricles of the adult human brain that proliferate in vivo and behave as multipotent progenitor cells in vitro. This astrocytic ribbon has not been observed in other vertebrates studied. Unexpectedly, we find no evidence of chains of migrating neuroblasts in the SVZ or in the pathway to the olfactory bulb. Our work identifies SVZ astrocytes as neural stem cells in a niche of unique organization in the adult human brain.
AB - The subventricular zone (SVZ) is a principal source of adult neural stem cells in the rodent brain, generating thousands of olfactory bulb neurons every day. If the adult human brain contains a comparable germinal region, this could have considerable implications for future neuroregenerative therapy. Stem cells have been isolated from the human brain, but the identity, organization and function of adult neural stem cells in the human SVZ are unknown. Here we describe a ribbon of SVZ astrocytes lining the lateral ventricles of the adult human brain that proliferate in vivo and behave as multipotent progenitor cells in vitro. This astrocytic ribbon has not been observed in other vertebrates studied. Unexpectedly, we find no evidence of chains of migrating neuroblasts in the SVZ or in the pathway to the olfactory bulb. Our work identifies SVZ astrocytes as neural stem cells in a niche of unique organization in the adult human brain.
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U2 - 10.1038/nature02301
DO - 10.1038/nature02301
M3 - Article
C2 - 14973487
AN - SCOPUS:1442354188
SN - 0028-0836
VL - 427
SP - 740
EP - 744
JO - Nature
JF - Nature
IS - 6976
ER -