Ultraviolet laser-induced fluorescence of colonic tissue

Basic biology and diagnostic potential

K. T. Schomacker, J. K. Frisoli, C. C. Compton, Thomas J Flotte, J. M. Richter, N. S. Nishioka, T. F. Deutsch

Research output: Contribution to journalArticle

411 Citations (Scopus)

Abstract

Laser-induced fluorescence (LIF) of colonic tissue was examined both in vitro and in vivo to assess the ability of the technique to distinguish neoplastic from hyperplastic and normal tissue and to relate the LIF spectra to specific constituents of the colon. Spectra from 86 normal colonic sites, 35 hyperplastic polyps, 49 adenomatous polyps, and 7 adenocarcinomas were recorded both in vivo and in vitro. With 337-nm excitation, the fluorescence spectra all had peaks at 390 and 460 nm, believed to arise from collagen and NADH, and a minimum at 425 nm, consistent with absorption attributable to hemoglobin. The spectra of colonic tissue recorded both in vivo and in vitro are different, primarily in the NADH fluorescence component, which decays exponentially with time after resection. When normal colonic tissue is compared to hyperplastic or adenomatous polyps, the predominant changes in the fluorescence spectra are a decrease in collagen fluorescence and a slight increase in hemoglobin reabsorption. A multivariate linear regression (MVLR) analysis was used to distinguish neoplastic tissue from non-neoplastic tissue with a sensitivity, specificity, predictive value positive, and predictive value negative toward neoplastic tissue of 80%, 92%, 82%, and 91%, respectively. When the MVLR technique was used to distinguish neoplastic polyps from non-neoplastic polyps, values of 86%, 77%, 86%, and 77% respectively, were obtained. The data suggest that the LIF measurements sense changes in polyp morphology, rather than changes in fluorophores specific to polyps, and it is this change in morphology that leads indirectly to discrimination of polyps.

Original languageEnglish (US)
Pages (from-to)63-78
Number of pages16
JournalLasers in Surgery and Medicine
Volume12
Issue number1
StatePublished - 1992
Externally publishedYes

Fingerprint

Polyps
Lasers
Fluorescence
Adenomatous Polyps
NAD
Linear Models
Hemoglobins
Collagen
Colon
Adenocarcinoma
Regression Analysis
Sensitivity and Specificity
In Vitro Techniques

ASJC Scopus subject areas

  • Surgery

Cite this

Schomacker, K. T., Frisoli, J. K., Compton, C. C., Flotte, T. J., Richter, J. M., Nishioka, N. S., & Deutsch, T. F. (1992). Ultraviolet laser-induced fluorescence of colonic tissue: Basic biology and diagnostic potential. Lasers in Surgery and Medicine, 12(1), 63-78.

Ultraviolet laser-induced fluorescence of colonic tissue : Basic biology and diagnostic potential. / Schomacker, K. T.; Frisoli, J. K.; Compton, C. C.; Flotte, Thomas J; Richter, J. M.; Nishioka, N. S.; Deutsch, T. F.

In: Lasers in Surgery and Medicine, Vol. 12, No. 1, 1992, p. 63-78.

Research output: Contribution to journalArticle

Schomacker, KT, Frisoli, JK, Compton, CC, Flotte, TJ, Richter, JM, Nishioka, NS & Deutsch, TF 1992, 'Ultraviolet laser-induced fluorescence of colonic tissue: Basic biology and diagnostic potential', Lasers in Surgery and Medicine, vol. 12, no. 1, pp. 63-78.
Schomacker, K. T. ; Frisoli, J. K. ; Compton, C. C. ; Flotte, Thomas J ; Richter, J. M. ; Nishioka, N. S. ; Deutsch, T. F. / Ultraviolet laser-induced fluorescence of colonic tissue : Basic biology and diagnostic potential. In: Lasers in Surgery and Medicine. 1992 ; Vol. 12, No. 1. pp. 63-78.
@article{8c643f14c60949b2a87e96280801b7d4,
title = "Ultraviolet laser-induced fluorescence of colonic tissue: Basic biology and diagnostic potential",
abstract = "Laser-induced fluorescence (LIF) of colonic tissue was examined both in vitro and in vivo to assess the ability of the technique to distinguish neoplastic from hyperplastic and normal tissue and to relate the LIF spectra to specific constituents of the colon. Spectra from 86 normal colonic sites, 35 hyperplastic polyps, 49 adenomatous polyps, and 7 adenocarcinomas were recorded both in vivo and in vitro. With 337-nm excitation, the fluorescence spectra all had peaks at 390 and 460 nm, believed to arise from collagen and NADH, and a minimum at 425 nm, consistent with absorption attributable to hemoglobin. The spectra of colonic tissue recorded both in vivo and in vitro are different, primarily in the NADH fluorescence component, which decays exponentially with time after resection. When normal colonic tissue is compared to hyperplastic or adenomatous polyps, the predominant changes in the fluorescence spectra are a decrease in collagen fluorescence and a slight increase in hemoglobin reabsorption. A multivariate linear regression (MVLR) analysis was used to distinguish neoplastic tissue from non-neoplastic tissue with a sensitivity, specificity, predictive value positive, and predictive value negative toward neoplastic tissue of 80{\%}, 92{\%}, 82{\%}, and 91{\%}, respectively. When the MVLR technique was used to distinguish neoplastic polyps from non-neoplastic polyps, values of 86{\%}, 77{\%}, 86{\%}, and 77{\%} respectively, were obtained. The data suggest that the LIF measurements sense changes in polyp morphology, rather than changes in fluorophores specific to polyps, and it is this change in morphology that leads indirectly to discrimination of polyps.",
author = "Schomacker, {K. T.} and Frisoli, {J. K.} and Compton, {C. C.} and Flotte, {Thomas J} and Richter, {J. M.} and Nishioka, {N. S.} and Deutsch, {T. F.}",
year = "1992",
language = "English (US)",
volume = "12",
pages = "63--78",
journal = "Lasers in Surgery and Medicine",
issn = "0196-8092",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Ultraviolet laser-induced fluorescence of colonic tissue

T2 - Basic biology and diagnostic potential

AU - Schomacker, K. T.

AU - Frisoli, J. K.

AU - Compton, C. C.

AU - Flotte, Thomas J

AU - Richter, J. M.

AU - Nishioka, N. S.

AU - Deutsch, T. F.

PY - 1992

Y1 - 1992

N2 - Laser-induced fluorescence (LIF) of colonic tissue was examined both in vitro and in vivo to assess the ability of the technique to distinguish neoplastic from hyperplastic and normal tissue and to relate the LIF spectra to specific constituents of the colon. Spectra from 86 normal colonic sites, 35 hyperplastic polyps, 49 adenomatous polyps, and 7 adenocarcinomas were recorded both in vivo and in vitro. With 337-nm excitation, the fluorescence spectra all had peaks at 390 and 460 nm, believed to arise from collagen and NADH, and a minimum at 425 nm, consistent with absorption attributable to hemoglobin. The spectra of colonic tissue recorded both in vivo and in vitro are different, primarily in the NADH fluorescence component, which decays exponentially with time after resection. When normal colonic tissue is compared to hyperplastic or adenomatous polyps, the predominant changes in the fluorescence spectra are a decrease in collagen fluorescence and a slight increase in hemoglobin reabsorption. A multivariate linear regression (MVLR) analysis was used to distinguish neoplastic tissue from non-neoplastic tissue with a sensitivity, specificity, predictive value positive, and predictive value negative toward neoplastic tissue of 80%, 92%, 82%, and 91%, respectively. When the MVLR technique was used to distinguish neoplastic polyps from non-neoplastic polyps, values of 86%, 77%, 86%, and 77% respectively, were obtained. The data suggest that the LIF measurements sense changes in polyp morphology, rather than changes in fluorophores specific to polyps, and it is this change in morphology that leads indirectly to discrimination of polyps.

AB - Laser-induced fluorescence (LIF) of colonic tissue was examined both in vitro and in vivo to assess the ability of the technique to distinguish neoplastic from hyperplastic and normal tissue and to relate the LIF spectra to specific constituents of the colon. Spectra from 86 normal colonic sites, 35 hyperplastic polyps, 49 adenomatous polyps, and 7 adenocarcinomas were recorded both in vivo and in vitro. With 337-nm excitation, the fluorescence spectra all had peaks at 390 and 460 nm, believed to arise from collagen and NADH, and a minimum at 425 nm, consistent with absorption attributable to hemoglobin. The spectra of colonic tissue recorded both in vivo and in vitro are different, primarily in the NADH fluorescence component, which decays exponentially with time after resection. When normal colonic tissue is compared to hyperplastic or adenomatous polyps, the predominant changes in the fluorescence spectra are a decrease in collagen fluorescence and a slight increase in hemoglobin reabsorption. A multivariate linear regression (MVLR) analysis was used to distinguish neoplastic tissue from non-neoplastic tissue with a sensitivity, specificity, predictive value positive, and predictive value negative toward neoplastic tissue of 80%, 92%, 82%, and 91%, respectively. When the MVLR technique was used to distinguish neoplastic polyps from non-neoplastic polyps, values of 86%, 77%, 86%, and 77% respectively, were obtained. The data suggest that the LIF measurements sense changes in polyp morphology, rather than changes in fluorophores specific to polyps, and it is this change in morphology that leads indirectly to discrimination of polyps.

UR - http://www.scopus.com/inward/record.url?scp=0026603020&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026603020&partnerID=8YFLogxK

M3 - Article

VL - 12

SP - 63

EP - 78

JO - Lasers in Surgery and Medicine

JF - Lasers in Surgery and Medicine

SN - 0196-8092

IS - 1

ER -