Ultrastructural localization of viral antigens in the CNS of mice persistently infected with lymphocytic choriomeningitis virus (LCMV)

M. Rodriguez, M. J. Buchmeier, M. B A Oldstone, P. W. Lampert

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Lymphocytic choriomeningitis virus (LCMV) produces a persistent infection of the nervous system in susceptible mice. To map the localization of viral antigens in the central nervous system (CNS), the authors studied, by means of ultrastructural immune peroxidase techniques, 4-6 month-old mice persistently infected with LCMV following an intracerebral inoculation at birth. The greatest number of infected neurons was observed in the cortex, particularly of the limbic system, and certain nuclei of the hypothalamus. In the cerebellum, Purkinje cells selectively expressed viral antigens. Moderate numbers of infected neurons were found in the anterior horns of the spinal cord, basal ganglia, and thalamus. The immunoperoxidase technique using monoclonal antibodies showed that persistently infected neurons primarily expressed the nucleocapsid protein antigens of LCMV. Glycopeptide antigens were minimally expressed. Electron-microscopic examination of selected individual infected neurons showed viral antigens exclusively associated with ribosomes. No staining was seen on cell surfaces. Glutaraldehyde-fixed CNS tissue studied by electron microscopy did not reveal morphologic abnormalities or mature viral particles. This study demonstrates that LCMV persistently infects specific neuronal populations. Infected neurons express viral antigens in association with host ribosomes and show no significant morphologic alterations.

Original languageEnglish (US)
Pages (from-to)95-100
Number of pages6
JournalAmerican Journal of Pathology
Volume110
Issue number1
StatePublished - 1983
Externally publishedYes

Fingerprint

Neurologic Mutant Mice
Lymphocytic choriomeningitis virus
Viral Antigens
Central Nervous System
Neurons
Ribosomes
Nucleocapsid Proteins
Antigens
Nerve Tissue
Limbic System
Glycopeptides
Purkinje Cells
Glutaral
Basal Ganglia
Thalamus
Immunoenzyme Techniques
Virion
Cerebellum
Nervous System
Peroxidase

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Ultrastructural localization of viral antigens in the CNS of mice persistently infected with lymphocytic choriomeningitis virus (LCMV). / Rodriguez, M.; Buchmeier, M. J.; Oldstone, M. B A; Lampert, P. W.

In: American Journal of Pathology, Vol. 110, No. 1, 1983, p. 95-100.

Research output: Contribution to journalArticle

Rodriguez, M. ; Buchmeier, M. J. ; Oldstone, M. B A ; Lampert, P. W. / Ultrastructural localization of viral antigens in the CNS of mice persistently infected with lymphocytic choriomeningitis virus (LCMV). In: American Journal of Pathology. 1983 ; Vol. 110, No. 1. pp. 95-100.
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