Ubiquitylation of FACT by the Cullin-E3 ligase Rtt101 connects FACT to DNA replication

Junhong Han, Qing Li, Laura McCullough, Charisse Kettelkamp, Tim Formosa, Zhiguo Zhang

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

FACT plays important roles in both gene transcription and DNA replication. However, how this protein complex is targeted to these two distinct cellular processes remains largely unknown. Here we show that ubiquitylation of the Spt16 subunit of FACT by Rtt101, the cullin subunit of an E3 ubiquitin ligase in Saccharomyces cerevisiae, links FACT to DNA replication. We find Rtt101 interacts with and ubiquitylates Spt16 in vitro and in vivo. Deletion of RTT101 leads to reduced association of both FACT and the replicative helicase MCM with replication origins. Loss of Rtt101 also reduces binding of FACT to MCM, but not the association of FACT with Leo1 and Spt5, two proteins involved in transcription. Origin function is compromised in cells lacking Rtt101 or with an Spt16 mutation. These findings identify Spt16 as an Rtt101 substrate, and suggest that Spt16 ubiquitylation is important for FACT to function during DNA replication.

Original languageEnglish (US)
Pages (from-to)1485-1490
Number of pages6
JournalGenes and Development
Volume24
Issue number14
DOIs
StatePublished - Jul 15 2010

Fingerprint

Cullin Proteins
Ubiquitin-Protein Ligases
Ubiquitination
DNA Replication
Replication Origin
Saccharomyces cerevisiae
Proteins
Mutation
Genes

Keywords

  • Chromatin
  • DNA replication
  • FACT
  • Gene transcription
  • Ubiquitylation

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Han, J., Li, Q., McCullough, L., Kettelkamp, C., Formosa, T., & Zhang, Z. (2010). Ubiquitylation of FACT by the Cullin-E3 ligase Rtt101 connects FACT to DNA replication. Genes and Development, 24(14), 1485-1490. https://doi.org/10.1101/gad.1887310

Ubiquitylation of FACT by the Cullin-E3 ligase Rtt101 connects FACT to DNA replication. / Han, Junhong; Li, Qing; McCullough, Laura; Kettelkamp, Charisse; Formosa, Tim; Zhang, Zhiguo.

In: Genes and Development, Vol. 24, No. 14, 15.07.2010, p. 1485-1490.

Research output: Contribution to journalArticle

Han, J, Li, Q, McCullough, L, Kettelkamp, C, Formosa, T & Zhang, Z 2010, 'Ubiquitylation of FACT by the Cullin-E3 ligase Rtt101 connects FACT to DNA replication', Genes and Development, vol. 24, no. 14, pp. 1485-1490. https://doi.org/10.1101/gad.1887310
Han J, Li Q, McCullough L, Kettelkamp C, Formosa T, Zhang Z. Ubiquitylation of FACT by the Cullin-E3 ligase Rtt101 connects FACT to DNA replication. Genes and Development. 2010 Jul 15;24(14):1485-1490. https://doi.org/10.1101/gad.1887310
Han, Junhong ; Li, Qing ; McCullough, Laura ; Kettelkamp, Charisse ; Formosa, Tim ; Zhang, Zhiguo. / Ubiquitylation of FACT by the Cullin-E3 ligase Rtt101 connects FACT to DNA replication. In: Genes and Development. 2010 ; Vol. 24, No. 14. pp. 1485-1490.
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