Tyrosine sulfation of human trypsin steers S2′ subsite selectivity towards basic amino acids

András Szabó, Moh'd A. Salameh, Maren Ludwig, Evette S Radisky, Miklós Sahin-Tóth

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Human cationic and anionic trypsins are sulfated on Tyr154, a residue which helps to shape the prime side substratebinding subsites. Here, we used phage display technology to assess the significance of tyrosine sulfation for the specificity of human trypsins. The prime side residues P1′-P4′ in the binding loop of bovine pancreatic trypsin inhibitor (BPTI) were fully randomized and tight binding inhibitor phages were selected against non-sulfated and sulfated human cationic trypsin. The selection pattern for the two targets differed mostly at the P2′ position, where variants selected against non-sulfated trypsin contained primarily aliphatic residues (Leu, Ile, Met), while variants selected against sulfated trypsin were enriched also for Arg. BPTI variants carrying Arg, Lys, Ile, Leu or Ala at the P2′ position of the binding loop were purified and equilibrium dissociation constants were determined against non-sulfated and sulfated cationic and anionic human trypsins. BPTI variants harboring apolar residues at P2′ exhibited 3-12-fold lower affinity to sulfated trypsin relative to the nonsulfated enzyme, whereas BPTI variants containing basic residues at P2′ had comparable affinity to both trypsin forms. Taken together, the observations demonstrate that the tyrosyl sulfate in human trypsins interacts with the P2′ position of the substrate-like inhibitor and this modification increases P2′ selectivity towards basic side chains.

Original languageEnglish (US)
Article numbere102063
JournalPLoS One
Volume9
Issue number7
DOIs
StatePublished - Jul 10 2014

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Basic Amino Acids
Trypsin
trypsin
Tyrosine
tyrosine
amino acids
Aprotinin
trypsin inhibitors
Bacteriophages
cattle
bacteriophages
Sulfates
sulfates
Display devices
Technology

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Tyrosine sulfation of human trypsin steers S2′ subsite selectivity towards basic amino acids. / Szabó, András; Salameh, Moh'd A.; Ludwig, Maren; Radisky, Evette S; Sahin-Tóth, Miklós.

In: PLoS One, Vol. 9, No. 7, e102063, 10.07.2014.

Research output: Contribution to journalArticle

Szabó, András ; Salameh, Moh'd A. ; Ludwig, Maren ; Radisky, Evette S ; Sahin-Tóth, Miklós. / Tyrosine sulfation of human trypsin steers S2′ subsite selectivity towards basic amino acids. In: PLoS One. 2014 ; Vol. 9, No. 7.
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