TY - JOUR
T1 - Tyrosine 110 in the measles virus phosphoprotein is required to block STAT1 phosphorylation
AU - Devaux, Patricia
AU - von Messling, Veronika
AU - Songsungthong, Warangkhana
AU - Springfeld, Christoph
AU - Cattaneo, Roberto
N1 - Funding Information:
We thank D. Gerlier for providing the anti-P antibody, S. Vongpunsawad for excellent technical support, and C. Samuel for helpful comments on the manuscript. This work was supported by NIH grants R01 AI 57761 and R01 AI 63476, and by a grant of the Mayo Foundation.
PY - 2007/3/30
Y1 - 2007/3/30
N2 - The measles virus (MV) P gene encodes three proteins: P, an essential polymerase cofactor, and C and V, which have multiple functions including immune evasion. We show here that the MV P protein also contributes to immune evasion, and that tyrosine 110 is required to block nuclear translocation of the signal transducer and activator of transcription factors (STAT) after interferon type I treatment. In particular, MV P inhibits STAT1 phosphorylation. This is shown not only by transient expression but also by reverse genetic analyses based on a new functional infectious cDNA derived from a MV vaccine vial (Moraten strain). Our study also identifies a conserved sequence around P protein tyrosine 110 as a candidate interaction site with a cellular protein.
AB - The measles virus (MV) P gene encodes three proteins: P, an essential polymerase cofactor, and C and V, which have multiple functions including immune evasion. We show here that the MV P protein also contributes to immune evasion, and that tyrosine 110 is required to block nuclear translocation of the signal transducer and activator of transcription factors (STAT) after interferon type I treatment. In particular, MV P inhibits STAT1 phosphorylation. This is shown not only by transient expression but also by reverse genetic analyses based on a new functional infectious cDNA derived from a MV vaccine vial (Moraten strain). Our study also identifies a conserved sequence around P protein tyrosine 110 as a candidate interaction site with a cellular protein.
KW - Innate immunity
KW - Interferon signaling
KW - Measles virus
KW - Phosphoprotein
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U2 - 10.1016/j.virol.2006.09.049
DO - 10.1016/j.virol.2006.09.049
M3 - Article
C2 - 17112561
AN - SCOPUS:33847329279
SN - 0042-6822
VL - 360
SP - 72
EP - 83
JO - Virology
JF - Virology
IS - 1
ER -