Type I diabetes mellitus does not alter initial splanchnic glucose extraction or hepatic UDP-glucose flux during enteral glucose administration

A. Vella, P. Shah, Rita Basu, A. Basu, M. Camilleri, W. F. Schwenk, R. A. Rizza

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Aims/hypothesis. Our aim was to determine whether an alteration in splanchnic glucose metabolism could contribute to postprandial hyperglycaemia in people with Type I (insulin-dependent) diabetes mellitus. Methods. Splanchnic glucose extraction, hepatic glycogen synthesis and endogenous glucose production were compared in 8 Type I diabetic patients and in 11 control subjects. Endogenous hormone secretion was inhibited with somatostatin while insulin (∼ 550 pmol/1) and glucagon (∼130 ng/1) concentrations were matched with exogenous hormone infusions. Glucose containing [3-3H] glucose was infused into the duodenum at a rate of 20 μmol ·. kg-1 · min-1 Plasma glucose concentrations were maintained at about 8.5 mmol/1 in both groups by means of a separate variable intravenous glucose infusion. Results. Initial splanchnic glucose uptake, calculated by subtracting the systemic rate of appearance of [3-3H] glucose from the rate of infusion of [3-3H] glucose into the duodenum, did not differ in the diabetic and non-diabetic patients (4.1 ± 0.8 vs 3.0 ± 1.0 μmol/kg/min). In addition, hepatic glycogen synthesis, measured using the acetaminophen glucuronide method did not differ (10.7 ± 2.4 vs 10.1 ± 2.7 μmol · kg-1 · min-1). On the other hand, suppression of endogenous glucose production, measured by an intravenous infusion of [6,6-2H2] glucose, was greater (p < 0.05) in the diabetic than in the non-diabetic subjects (1.7 ± 1.6 vs 5.8 ± 1.9 μmol · kg-1 · min-1). Conclusion/interpretation. When glucose, insulin and glucagon concentrations are matched in individuals with relatively good chronic glycaemic control, Type I diabetes does not alter initial splanchnic glucose uptake of enterally delivered glucose or hepatic glycogen synthesis. Alterations in splanchnic glucose metabolism are not likely to contribute to postprandial hyperglycaemia in people with well controlled Type I diabetes.

Original languageEnglish (US)
Pages (from-to)729-737
Number of pages9
JournalDiabetologia
Volume44
Issue number6
DOIs
StatePublished - Jan 1 2001

Keywords

  • Acetaminophen glucuronide
  • Hepatic glycogen synthesis
  • Postprandial hyperglycaemia
  • Splanchnic glucose metabolism
  • Type I diabetes mellitus

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

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