TY - JOUR
T1 - Type Iγ661 phosphatidylinositol phosphate kinase directly interacts with AP2 and regulates endocytosis
AU - Bairstow, Shawn F.
AU - Ling, Kun
AU - Su, Xiaojing
AU - Firestone, Ari J.
AU - Carbonara, Chateen
AU - Anderson, Richard A.
PY - 2006/7/21
Y1 - 2006/7/21
N2 - Clathrin-coated vesicles mediate sorting and intracellular transport of membrane-bound proteins. The formation of these coats is initiated by the assembly of adaptor proteins (AP), which specifically bind to membrane cargo proteins via recognition of endocytic sorting motifs. The lipid signaling molecule phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is critical for this process, as it serves as both a targeting and regulatory factor. PI(4,5)P2 is synthesized by type I phosphatidylinositol phosphate kinases (PIPKI). We have discovered a direct interaction between the μ2-subunit of the AP2 complex and PIPKIγ661 via a yeast two-hybrid screen. This interaction was confirmed using both the μ2-subunit in glutathione S-transferase pulldowns and via coimmunoprecipitation of endogenous PIPKIγ661 with the AP2 complex from HEK293 cells. The interaction is mediated, in vivo, by a tyrosine-based motif in the 26-amino acid tail of PIPKIγ661. Because AP2 regulates endocytosis of transferrin receptor from the plasma membrane, we also examined a role for PIPKIγ661 using a flow cytometry endocytosis assay. We observed that stable expression of wild type PIPKIγ661 in Madin-Darby canine kidney cells enhanced transferrin uptake, whereas stable expression of kinase-dead PIPKIγ661 had an inhibitory effect. Neither condition affected the overall cellular level of PI(4,5)P 2. RNA interference-based knockdown of PIPKIγ661 in HeLa cells also had an inhibitory effect on transferrin endocytosis using the same assay system. Collectively, this evidence implies an important role for PIPKIγ661 in the AP2-mediated endocytosis of transferrin.
AB - Clathrin-coated vesicles mediate sorting and intracellular transport of membrane-bound proteins. The formation of these coats is initiated by the assembly of adaptor proteins (AP), which specifically bind to membrane cargo proteins via recognition of endocytic sorting motifs. The lipid signaling molecule phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is critical for this process, as it serves as both a targeting and regulatory factor. PI(4,5)P2 is synthesized by type I phosphatidylinositol phosphate kinases (PIPKI). We have discovered a direct interaction between the μ2-subunit of the AP2 complex and PIPKIγ661 via a yeast two-hybrid screen. This interaction was confirmed using both the μ2-subunit in glutathione S-transferase pulldowns and via coimmunoprecipitation of endogenous PIPKIγ661 with the AP2 complex from HEK293 cells. The interaction is mediated, in vivo, by a tyrosine-based motif in the 26-amino acid tail of PIPKIγ661. Because AP2 regulates endocytosis of transferrin receptor from the plasma membrane, we also examined a role for PIPKIγ661 using a flow cytometry endocytosis assay. We observed that stable expression of wild type PIPKIγ661 in Madin-Darby canine kidney cells enhanced transferrin uptake, whereas stable expression of kinase-dead PIPKIγ661 had an inhibitory effect. Neither condition affected the overall cellular level of PI(4,5)P 2. RNA interference-based knockdown of PIPKIγ661 in HeLa cells also had an inhibitory effect on transferrin endocytosis using the same assay system. Collectively, this evidence implies an important role for PIPKIγ661 in the AP2-mediated endocytosis of transferrin.
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U2 - 10.1074/jbc.M601465200
DO - 10.1074/jbc.M601465200
M3 - Article
C2 - 16707488
AN - SCOPUS:33745984211
SN - 0021-9258
VL - 281
SP - 20632
EP - 20642
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 29
ER -