TY - JOUR
T1 - Two target sites for protein binding in the promoter region of a cell cycle regulated human H1 histone gene
AU - van Wijnen, Andre J.
AU - Wright, Kenneth L.
AU - Massung, Robert F.
AU - Gerretsen, Martijn
AU - Stein, Janet L.
AU - Stein, Gary S.
N1 - Funding Information:
This work was supported by grants from the National Institute of Health (GM 32010, Ot 32381), the National Science Foundation (PCM 83-18177) and the March of Dimes Birth Defects Foundation (1-183). AJvW and MS are recipients of an exchange program grant from the University of Utrecht, Netherlands, under the supervision of Dr. Harry Voarma. We thank Wesley Faunce for performing initial experiments and Drs. Harry Nick, Peter van der Vliet and Erik de Vries for advice and materials. Special thanks to Urs Paul! and Susan Qirysogelos far critical discussions.
PY - 1988/1/25
Y1 - 1988/1/25
N2 - The 5' region of a cell cycle regulated human H1 histone gene appears to contain at least six promoter DNA elements that are shared with some, but not all human core (H2A, H2B, H3 and H4) histone genes. We show that two of these elements represent separate binding sites for two distinct, partially purified factors. The first promoter domain contains A/T rich repeats and is involved in the binding of HiNF-A, a nuclear factor previously found to bind to A/T rich direct repeats in the promoters of human H4 and H3 histone genes. The second domain, containing the general promoter element 5' dACCAAT, acts as a binding site for a two component mosaic factor we have designated HiNF-B. These data suggest that coordinate transcriptional regulation of human H1 and core histone genes may involve two classes of trans-acting factors: those specific far histone gene promoters and those that act on a broad spectrum of human gene promoters.
AB - The 5' region of a cell cycle regulated human H1 histone gene appears to contain at least six promoter DNA elements that are shared with some, but not all human core (H2A, H2B, H3 and H4) histone genes. We show that two of these elements represent separate binding sites for two distinct, partially purified factors. The first promoter domain contains A/T rich repeats and is involved in the binding of HiNF-A, a nuclear factor previously found to bind to A/T rich direct repeats in the promoters of human H4 and H3 histone genes. The second domain, containing the general promoter element 5' dACCAAT, acts as a binding site for a two component mosaic factor we have designated HiNF-B. These data suggest that coordinate transcriptional regulation of human H1 and core histone genes may involve two classes of trans-acting factors: those specific far histone gene promoters and those that act on a broad spectrum of human gene promoters.
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U2 - 10.1093/nar/16.2.571
DO - 10.1093/nar/16.2.571
M3 - Article
C2 - 2829131
AN - SCOPUS:0023884084
SN - 0305-1048
VL - 16
SP - 571
EP - 592
JO - Nucleic acids research
JF - Nucleic acids research
IS - 2
ER -