Abstract
A new two-step cycle PCR method has been developed for amplification of GC-rich DNA sequences. Using this method, termed 'hot PCR', 111 and 179 bp regions (GC contents of 74% and 76%, respectively) of the avian c-myc proto-oncogene were specifically amplified from cloned and genomic DNA. This method uses high-melting primers (T(m) between 70° and 74°C), a two-step cycle that employs a 94°C denaturation step and an annealing-elongation step between 70° and 80°C with or without formamide.
Original language | English (US) |
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Pages (from-to) | 390-392+394 |
Journal | BioTechniques |
Volume | 14 |
Issue number | 3 |
State | Published - 1993 |
ASJC Scopus subject areas
- Biotechnology
- General Biochemistry, Genetics and Molecular Biology