Two-step 'hot' PCR amplification of GC-rich avian c-myc sequences

M. Schuchard; G. Sarkar; T. Ruesink; T. C. Spelsberg

Two-step 'hot' PCR amplification of GC-rich avian c-myc sequences

M. Schuchard, G. Sarkar, T. Ruesink, T. C. Spelsberg

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A new two-step cycle PCR method has been developed for amplification of GC-rich DNA sequences. Using this method, termed 'hot PCR', 111 and 179 bp regions (GC contents of 74% and 76%, respectively) of the avian c-myc proto-oncogene were specifically amplified from cloned and genomic DNA. This method uses high-melting primers (T(m) between 70° and 74°C), a two-step cycle that employs a 94°C denaturation step and an annealing-elongation step between 70° and 80°C with or without formamide.

Original language	English (US)
Pages (from-to)	390-392+394
Journal	BioTechniques
Volume	14
Issue number	3
State	Published - 1993

ASJC Scopus subject areas

Biotechnology
General Biochemistry, Genetics and Molecular Biology

Cite this

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title = "Two-step 'hot' PCR amplification of GC-rich avian c-myc sequences",

abstract = "A new two-step cycle PCR method has been developed for amplification of GC-rich DNA sequences. Using this method, termed 'hot PCR', 111 and 179 bp regions (GC contents of 74% and 76%, respectively) of the avian c-myc proto-oncogene were specifically amplified from cloned and genomic DNA. This method uses high-melting primers (T(m) between 70° and 74°C), a two-step cycle that employs a 94°C denaturation step and an annealing-elongation step between 70° and 80°C with or without formamide.",

author = "M. Schuchard and G. Sarkar and T. Ruesink and Spelsberg, {T. C.}",

year = "1993",

language = "English (US)",

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T1 - Two-step 'hot' PCR amplification of GC-rich avian c-myc sequences

AU - Schuchard, M.

AU - Sarkar, G.

AU - Ruesink, T.

AU - Spelsberg, T. C.

PY - 1993

Y1 - 1993

N2 - A new two-step cycle PCR method has been developed for amplification of GC-rich DNA sequences. Using this method, termed 'hot PCR', 111 and 179 bp regions (GC contents of 74% and 76%, respectively) of the avian c-myc proto-oncogene were specifically amplified from cloned and genomic DNA. This method uses high-melting primers (T(m) between 70° and 74°C), a two-step cycle that employs a 94°C denaturation step and an annealing-elongation step between 70° and 80°C with or without formamide.

AB - A new two-step cycle PCR method has been developed for amplification of GC-rich DNA sequences. Using this method, termed 'hot PCR', 111 and 179 bp regions (GC contents of 74% and 76%, respectively) of the avian c-myc proto-oncogene were specifically amplified from cloned and genomic DNA. This method uses high-melting primers (T(m) between 70° and 74°C), a two-step cycle that employs a 94°C denaturation step and an annealing-elongation step between 70° and 80°C with or without formamide.

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ER -

Two-step 'hot' PCR amplification of GC-rich avian c-myc sequences

Abstract

ASJC Scopus subject areas

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