Two preadipocyte subtypes cloned from human omental fat.

James L Kirkland, C. H. Hollenberg, W. S. Gillon

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

To determine whether the characteristics of preadipocytes derived from human fat are uniform or variable, we developed methods for culturing and differentiating cloned human preadipocytes. Individual human omental preadipocytes were cultured for six weeks. The number of cells varied considerably among clones derived from the same subject, implying that human preadipocytes vary in replicative capacity. Indeed, two cell subtypes were found in human omental fat; one type replicated slowly and the other was capable of extensive replication. Cells of both subtypes were capable of differentiation into adipocytes, confirming that both subtypes were preadipocytes. When rat perirenal and epididymal preadipocytes were cloned, a slowly replicating and an extensively replicating preadipocyte subtype were also found. It is proposed that preadipocytes of the rapidly and the slowly replicating subtypes may be at different stages along the pathway between uncommitted precursor cells and differentiated adipocytes.

Original languageEnglish (US)
Pages (from-to)87-91
Number of pages5
JournalObesity research.
Volume1
Issue number2
StatePublished - Mar 1993
Externally publishedYes

Fingerprint

Fats
lipids
adipocytes
Adipocytes
cells
Clone Cells
Cell Count
clones
rats
methodology

ASJC Scopus subject areas

  • Food Science
  • Endocrinology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Public Health, Environmental and Occupational Health

Cite this

Kirkland, J. L., Hollenberg, C. H., & Gillon, W. S. (1993). Two preadipocyte subtypes cloned from human omental fat. Obesity research., 1(2), 87-91.

Two preadipocyte subtypes cloned from human omental fat. / Kirkland, James L; Hollenberg, C. H.; Gillon, W. S.

In: Obesity research., Vol. 1, No. 2, 03.1993, p. 87-91.

Research output: Contribution to journalArticle

Kirkland, JL, Hollenberg, CH & Gillon, WS 1993, 'Two preadipocyte subtypes cloned from human omental fat.', Obesity research., vol. 1, no. 2, pp. 87-91.
Kirkland, James L ; Hollenberg, C. H. ; Gillon, W. S. / Two preadipocyte subtypes cloned from human omental fat. In: Obesity research. 1993 ; Vol. 1, No. 2. pp. 87-91.
@article{10cc815aa56a4307b7029de1690165cc,
title = "Two preadipocyte subtypes cloned from human omental fat.",
abstract = "To determine whether the characteristics of preadipocytes derived from human fat are uniform or variable, we developed methods for culturing and differentiating cloned human preadipocytes. Individual human omental preadipocytes were cultured for six weeks. The number of cells varied considerably among clones derived from the same subject, implying that human preadipocytes vary in replicative capacity. Indeed, two cell subtypes were found in human omental fat; one type replicated slowly and the other was capable of extensive replication. Cells of both subtypes were capable of differentiation into adipocytes, confirming that both subtypes were preadipocytes. When rat perirenal and epididymal preadipocytes were cloned, a slowly replicating and an extensively replicating preadipocyte subtype were also found. It is proposed that preadipocytes of the rapidly and the slowly replicating subtypes may be at different stages along the pathway between uncommitted precursor cells and differentiated adipocytes.",
author = "Kirkland, {James L} and Hollenberg, {C. H.} and Gillon, {W. S.}",
year = "1993",
month = "3",
language = "English (US)",
volume = "1",
pages = "87--91",
journal = "Obesity",
issn = "1930-7381",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Two preadipocyte subtypes cloned from human omental fat.

AU - Kirkland, James L

AU - Hollenberg, C. H.

AU - Gillon, W. S.

PY - 1993/3

Y1 - 1993/3

N2 - To determine whether the characteristics of preadipocytes derived from human fat are uniform or variable, we developed methods for culturing and differentiating cloned human preadipocytes. Individual human omental preadipocytes were cultured for six weeks. The number of cells varied considerably among clones derived from the same subject, implying that human preadipocytes vary in replicative capacity. Indeed, two cell subtypes were found in human omental fat; one type replicated slowly and the other was capable of extensive replication. Cells of both subtypes were capable of differentiation into adipocytes, confirming that both subtypes were preadipocytes. When rat perirenal and epididymal preadipocytes were cloned, a slowly replicating and an extensively replicating preadipocyte subtype were also found. It is proposed that preadipocytes of the rapidly and the slowly replicating subtypes may be at different stages along the pathway between uncommitted precursor cells and differentiated adipocytes.

AB - To determine whether the characteristics of preadipocytes derived from human fat are uniform or variable, we developed methods for culturing and differentiating cloned human preadipocytes. Individual human omental preadipocytes were cultured for six weeks. The number of cells varied considerably among clones derived from the same subject, implying that human preadipocytes vary in replicative capacity. Indeed, two cell subtypes were found in human omental fat; one type replicated slowly and the other was capable of extensive replication. Cells of both subtypes were capable of differentiation into adipocytes, confirming that both subtypes were preadipocytes. When rat perirenal and epididymal preadipocytes were cloned, a slowly replicating and an extensively replicating preadipocyte subtype were also found. It is proposed that preadipocytes of the rapidly and the slowly replicating subtypes may be at different stages along the pathway between uncommitted precursor cells and differentiated adipocytes.

UR - http://www.scopus.com/inward/record.url?scp=0342463641&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0342463641&partnerID=8YFLogxK

M3 - Article

C2 - 16350564

AN - SCOPUS:0342463641

VL - 1

SP - 87

EP - 91

JO - Obesity

JF - Obesity

SN - 1930-7381

IS - 2

ER -