TY - JOUR
T1 - Two-dimensional digital video ankle motion analysis for assessment of function in the rat sciatic nerve model
AU - De Ruiter, Godard C.
AU - Spinner, Robert J.
AU - Alaid, Awad O.
AU - Koch, Anthony J.
AU - Wang, Huan
AU - Malessy, Martijn J.A.
AU - Currier, Bradford L.
AU - Yaszemski, Michael J.
AU - Kaufman, Kenton R.
AU - Windebank, Anthony J.
PY - 2007/9
Y1 - 2007/9
N2 - Ankle motion analysis may provide a better method to assess function in the rat sciatic nerve model than the standard method, the sciatic functional index (SFI), but it is not widely used in experiments on nerve regeneration possibly because of complicated analysis. In this study, we investigated the practical use of a two-dimensional (2D) digital video motion analysis system. Reproducibility was investigated in normal rats. Recovery of ankle motion was analyzed after sciatic, tibial, and peroneal nerve crush injury. Results were compared with scores for the SFI. Results were not significantly different from animal-to-animal and day-to-day. Interobserver variability also was small. In the analysis of recovery after separate nerve crush injuries, subtle differences in ankle plantar flexion and dorsiflexion could be detected. The method was also more sensitive than the SFI: whereas scores for the SFI had returned to normal 4 weeks after sciatic nerve crush injury, the ankle angle at mid-stance was still significantly different from that in sham-operated animals 6 weeks after the injury. 2D digital video ankle motion analysis is a practical and sensitive method to assess function in the rat sciatic nerve model.
AB - Ankle motion analysis may provide a better method to assess function in the rat sciatic nerve model than the standard method, the sciatic functional index (SFI), but it is not widely used in experiments on nerve regeneration possibly because of complicated analysis. In this study, we investigated the practical use of a two-dimensional (2D) digital video motion analysis system. Reproducibility was investigated in normal rats. Recovery of ankle motion was analyzed after sciatic, tibial, and peroneal nerve crush injury. Results were compared with scores for the SFI. Results were not significantly different from animal-to-animal and day-to-day. Interobserver variability also was small. In the analysis of recovery after separate nerve crush injuries, subtle differences in ankle plantar flexion and dorsiflexion could be detected. The method was also more sensitive than the SFI: whereas scores for the SFI had returned to normal 4 weeks after sciatic nerve crush injury, the ankle angle at mid-stance was still significantly different from that in sham-operated animals 6 weeks after the injury. 2D digital video ankle motion analysis is a practical and sensitive method to assess function in the rat sciatic nerve model.
KW - Nerve injury
KW - Nerve regeneration
KW - Nerve repair
KW - Sciatic function index
KW - Walking track analysis
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U2 - 10.1111/j.1529-8027.2007.00142.x
DO - 10.1111/j.1529-8027.2007.00142.x
M3 - Article
C2 - 17868249
AN - SCOPUS:34548617374
SN - 1085-9489
VL - 12
SP - 216
EP - 222
JO - Journal of the Peripheral Nervous System
JF - Journal of the Peripheral Nervous System
IS - 3
ER -