Two different molecular defects in the Tva receptor gene explain the resistance of two tvar lines of chickens to infection by subgroup A avian sarcoma and leukosis viruses

Daniel Elleder, Deborah C. Melder, Katerina Trejbalova, Jan Svoboda, Mark J Federspiel

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The subgroup A to E avian sarcoma and leukosis viruses (ASLVs) are highly related and are thought to have evolved from a common ancestor. These viruses use distinct cell surface proteins as receptors to gain entry into avian cells. Chickens have evolved resistance to infection by the ASLVs. We have identified the mutations responsible for the block to virus entry in chicken lines resistant to infection by subgroup A ASLVs [ASLV(A)]. The tva genetic locus determines the susceptibility of chicken cells to ASLV(A) viruses. In quail, the ASLV(A) susceptibility allele tvaS encodes two forms of the Tva receptor; these proteins are translated from alternatively spliced mRNAs. The normal cellular function of the Tva receptor is unknown; however, the extracellular domain contains a 40-amino-acid, cysteine-rich region that is homologous to the ligand binding region of the low-density lipoprotein receptor (LDLR) proteins. The chicken tvaS cDNAs had not yet been fully characterized; we cloned the chicken tva cDNAs from two lines of subgroup A-susceptible chickens, line H6 and line 0. Two types of chicken tvaS cDNAs were obtained. These cDNAs encode a longer and shorter form of the Tva receptor homologous to the Tva forms in quail. Two different defects were identified in cDNAs cloned from two different ASLV(A)-resistant inbred chickens, line C and line 72.. Line C tvar contains a single base pair substitution, resulting in a cysteine-to-tryptophan change in the LDLR-like region of Tva. This mutation drastically reduces the binding affinity of TvaR for the ASLV(A) envelope glycoproteins. Line 72 tvar2 contains a 4-bp insertion in exon 1 that causes a change in the reading frame, which blocks expression of the Tva receptor.

Original languageEnglish (US)
Pages (from-to)13489-13500
Number of pages12
JournalJournal of Virology
Volume78
Issue number24
DOIs
StatePublished - Dec 2004

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Alpharetrovirus
Rous sarcoma virus
Avian leukosis virus
Chickens
chickens
receptors
Infection
Complementary DNA
infection
Genes
genes
Quail
LDL Receptors
low density lipoprotein
quails
viruses
Cysteine
cysteine
Viruses
mutation

ASJC Scopus subject areas

  • Immunology

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Two different molecular defects in the Tva receptor gene explain the resistance of two tvar lines of chickens to infection by subgroup A avian sarcoma and leukosis viruses. / Elleder, Daniel; Melder, Deborah C.; Trejbalova, Katerina; Svoboda, Jan; Federspiel, Mark J.

In: Journal of Virology, Vol. 78, No. 24, 12.2004, p. 13489-13500.

Research output: Contribution to journalArticle

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