TY - JOUR
T1 - Twelve-hour cardiopulmonary preservation using donor core cooling, leukocyte depletion, and liposomal superoxide dismutase
AU - Bando, K.
AU - Schueler, S.
AU - Cameron, D. E.
AU - DeValeria, P. A.
AU - Hatanaka, M.
AU - Casale, A. S.
AU - Zebley, M. A.
AU - Hutchins, G. M.
AU - Reitz, B. A.
AU - Baumgartner, W. A.
PY - 1991
Y1 - 1991
N2 - Because leukocytes and oxygen radical species contribute to ischemic and reperfusion injury during organ preservation, we examined the effect of a long-acting liposomal superoxide dismutase (liposomal SOD) and mechanical filtration of leukocytes on cardiopulmonary graft function after 12 hours of static preservation. Bovine heart-lung blocks were harvested, core cooled to 15°C, stored in 4°C donor blood for 12 hours, and then orthotopically transplanted (control group, n = 6). In the leukocyte-depletion group (n = 6), a leukocyte filter was incorporated in the bypass circuits of the donor and recipient. In the SOD group (n = 6), liposomal SOD (5000 U/kg) was administered in the cardioplegic solution, in the prime of the bypass circuits of donor and recipient, and immediately before recipient heart-lung reperfusion. In the combination group (n = 6), both leukocyte depletion (LD) and liposomal SOD were used. Only four of six control animals survived more than 2 hours after weaning from bypass, whereas all LD, SOD, and LD + SOD animals survived to be studied at 6 hours. Pulmonary function was assessed at 6 hours by arterial oxygen tension on 100% inspired oxygen (pO2), pulmonary vascular resistance (PVR), and postmortem wet/dry lung weight ratios. Arterial pO2 values (mm Hg) were as follows: control, 102 ± 51; LD, 437 ± 60*; SOD, 278 ± 83; and LD + SOD, 504 ± 54* (*p < 0.05 vs controls). PVR values (dynes · sec · cm5) were as follows: control, 1975 ± 697; LD, 682 ± 131*; SOD, 607 ± 191 *; and LD + SOD 367 ± 87* (*p < 0.05 vs controls). Postmortem wet/dry lung weight ratios were as follows: control, 7.2 ± 0.3; LD, 6.3 ± 0.2; SOD, 6.3 ± 0.3; and LD + SOD, 4.7 ± 0.3* (*p < 0.05 vs controls). Donor core cooling and leukocyte depletion combined with liposomal SOD provided the best protection. With this technique, extended cardiopulmonary preservation for heart-lung transplantation may be achieved
AB - Because leukocytes and oxygen radical species contribute to ischemic and reperfusion injury during organ preservation, we examined the effect of a long-acting liposomal superoxide dismutase (liposomal SOD) and mechanical filtration of leukocytes on cardiopulmonary graft function after 12 hours of static preservation. Bovine heart-lung blocks were harvested, core cooled to 15°C, stored in 4°C donor blood for 12 hours, and then orthotopically transplanted (control group, n = 6). In the leukocyte-depletion group (n = 6), a leukocyte filter was incorporated in the bypass circuits of the donor and recipient. In the SOD group (n = 6), liposomal SOD (5000 U/kg) was administered in the cardioplegic solution, in the prime of the bypass circuits of donor and recipient, and immediately before recipient heart-lung reperfusion. In the combination group (n = 6), both leukocyte depletion (LD) and liposomal SOD were used. Only four of six control animals survived more than 2 hours after weaning from bypass, whereas all LD, SOD, and LD + SOD animals survived to be studied at 6 hours. Pulmonary function was assessed at 6 hours by arterial oxygen tension on 100% inspired oxygen (pO2), pulmonary vascular resistance (PVR), and postmortem wet/dry lung weight ratios. Arterial pO2 values (mm Hg) were as follows: control, 102 ± 51; LD, 437 ± 60*; SOD, 278 ± 83; and LD + SOD, 504 ± 54* (*p < 0.05 vs controls). PVR values (dynes · sec · cm5) were as follows: control, 1975 ± 697; LD, 682 ± 131*; SOD, 607 ± 191 *; and LD + SOD 367 ± 87* (*p < 0.05 vs controls). Postmortem wet/dry lung weight ratios were as follows: control, 7.2 ± 0.3; LD, 6.3 ± 0.2; SOD, 6.3 ± 0.3; and LD + SOD, 4.7 ± 0.3* (*p < 0.05 vs controls). Donor core cooling and leukocyte depletion combined with liposomal SOD provided the best protection. With this technique, extended cardiopulmonary preservation for heart-lung transplantation may be achieved
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M3 - Article
C2 - 2031929
AN - SCOPUS:0025843707
SN - 1053-2498
VL - 10
SP - 304
EP - 309
JO - Journal of Heart and Lung Transplantation
JF - Journal of Heart and Lung Transplantation
IS - 2
ER -