Tumor necrosis factor α and interleukin-1β mRNA expression in HMC-1 cells: Differential regulation of gene product expression by recombinant interleukin-4

C. Sillaber, D. Bevec, J. H. Butterfield, C. Heppner, R. Valenta, O. Scheiner, D. Kraft, K. Lechner, P. Bettelheim, P. Valent

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

Cytokine-activation pathways in mast cells are supposed to play a significant role in host defense mechanisms and allergic reactions. Interleukin-4 (IL-4) is a well-characterized regulator of growth and function of mast cells. The human mast cell line HMC-1 was established from a patient suffering from mast cell leukemia and was shown to expose IL-4 binding sites. In the present study, the effects of recombinant human (rh) IL-4 and other rh cytokines (IL-2, IL-3, IL-6, IL-8) on expression of cytokine mRNA in HMC-1 cells were examined by Northern blot analysis using oligonucleotide probes. Tumor necrosis factor α (TNF-α) and IL-1β transcripts were found to be expressed constitutively in HMC-1 cells, whereas transcripts for IL-3, IL-4, IL-5, IL-6, and granulocyte-macrophage colony-stimulating factor (GM-CSF) could not be detected. Of all cytokines tested, rhIL-4 was found to down-regulate IL-1β mRNA expression and formation of immunoreactive IL-1β protein in HMC-1 cells. The effect of IL-4 on IL-1β gene product expression was time- and dose-dependent (maximum effects obtained with 100 U/mL of rhIL-4). No effect of IL-4 on expression of TNF-α mRNA in HMC-1 cells was observed. These results raise the possibility that human mast cells are a source of both TNF-α and IL-1β. Furthermore, our study provides evidence that IL-4 regulates IL-1β gene product expression in HMC-1 cells. The HMC-1 cell line should be a useful tool for studying cytokine activation pathways in human mast cells.

Original languageEnglish (US)
Pages (from-to)1271-1275
Number of pages5
JournalExperimental Hematology
Volume21
Issue number9
StatePublished - Dec 1 1993

Keywords

  • Cytokines
  • Cytotoxicity
  • IL-1
  • IL-4
  • Mast cells

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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    Sillaber, C., Bevec, D., Butterfield, J. H., Heppner, C., Valenta, R., Scheiner, O., Kraft, D., Lechner, K., Bettelheim, P., & Valent, P. (1993). Tumor necrosis factor α and interleukin-1β mRNA expression in HMC-1 cells: Differential regulation of gene product expression by recombinant interleukin-4. Experimental Hematology, 21(9), 1271-1275.