Tumor and histocompatibility antigen interaction: direct visualization by a double-label bridging technique for immuno-electron microscopy

Randal E. Morris, Chella S. David, Michael D. Manhart

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2 Scopus citations


The nature of the interaction between the Ib tumor-associated surface antigen (Ib-TASA) and the Dk and Kk (Dk/Kk) regions of the major histocompatibility complex on the surface of line Ib lymphocytes was evaluated by immuno-electron microscopy using a double-label bridging technique. Quantitation of antigenic sites by direct counts showed 44% of the Ib-TASA sites coincident with Dk/Kk antigens. This is greater than the predicted random interaction of two independent cell surface determinants suggesting an associative interaction, i.e. 'altered-self' antigen. The remaining 56% of the Ib-TASA sites were in a non-associative mode. On the basis of data presented here, we postulate that the interaction between 'self'- and 'non-self' antigens is not an 'all or none' phenomenon.

Original languageEnglish (US)
Pages (from-to)973-982
Number of pages10
JournalMolecular Immunology
Issue number8
StatePublished - Aug 1982


ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

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