Tuberous sclerosis 1 (Tsc1)-dependent metabolic checkpoint controls development of dendritic cells

Yanyan Wang, Gonghua Huang, Hu Zeng, Kai Yang, Richard F. Lamb, Hongbo Chi

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49 Citations (Scopus)

Abstract

Coordination of cell metabolism and immune signals is crucial for lymphocyte priming. Emerging evidence also highlights the importance of cell metabolism for the activation of innate immunity upon pathogen challenge, but there is little evidence of how this process contributes to immune cell development. Here we show that differentiation of dendritic cells (DCs) from bone marrow precursors is associated with dynamic regulation of mechanistic target of rapamycin (mTOR) complex 1 (mTORC1) signaling and cell metabolism. Unexpectedly, enhancing mTORC1 activity via ablation of its negative regulator tuberous sclerosis 1 (Tsc1) impaired DC development in vivo and in vitro, associated with defective cell survival and proliferation. Moreover, Tsc1 deficiency caused DC spontaneous maturation but a propensity to differentiate into other lineages, and attenuated DC-mediated effector TH1 responses. Mechanistically, Tsc1-deficient DCs exhibited increased glycolysis, mitochondrial respiration, and lipid synthesis that were partly mediated by the transcription factor Myc, highlighting a key role of Tsc1 in modulating metabolic programming of DC differentiation. Further, Tsc1 signaled through Rheb to down-regulate mTORC1 for proper DC development, whereas its effect at modulating mTOR complex 2 (mTORC2) activity was largely dispensable. Our results demonstrate that the interplay between Tsc1-Rheb-mTORC1 signaling and Myc-dependent bioenergetic and biosynthetic activities constitutes a key metabolic checkpoint to orchestrate DC development.

Original languageEnglish (US)
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number50
DOIs
StatePublished - Dec 10 2013
Externally publishedYes

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Dendritic Cells
Tuberous Sclerosis 1
Glycolysis
Innate Immunity
Energy Metabolism
Cell Differentiation
Cell Survival
Respiration
Transcription Factors
Down-Regulation
Bone Marrow
Cell Proliferation
Lymphocytes
Lipids

ASJC Scopus subject areas

  • General

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Tuberous sclerosis 1 (Tsc1)-dependent metabolic checkpoint controls development of dendritic cells. / Wang, Yanyan; Huang, Gonghua; Zeng, Hu; Yang, Kai; Lamb, Richard F.; Chi, Hongbo.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 50, 10.12.2013.

Research output: Contribution to journalArticle

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