TH2-mediated pulmonary inflammation leads to the differential expression of ribonuclease genes by alveolar macrophages

Stephania A. Cormier, Shubing Yuan, Jeffrey R. Crosby, Cheryl A. Protheroe, Dawn M. Dimina, Edith M. Hines, Nancy A Lee, James J. Lee

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The eosinophil-associated ribonuclease (Ear) family in the mouse consists of thirteen genes, eleven of which encode RNases that have physical/functional properties similar to the human Ears, eosinophil-derived neurotoxin and eosinophil cationic protein. The expression of Ear genes in the mouse is confined to sites of known eosinophilopoiesis, with the exception of the lung. Two Ear genes, Ear1 and Ear2, are predominantly expressed in the lungs of naive mice. Total Ear gene expression and RNase activity in bronchoalveolar lavage fluid increases significantly upon the induction of pulmonary inflammation using an ovalbumin (OVA) model of allergic sensitization and challenge. Interestingly, pulmonary Ear11 transcripts, which are absent in naive mice, accumulate as a consequence of OVA-mediated TH2 inflammation in the lung. The induction of Ear11 expression is dependent on the presence of T cells, in particular, CD4+ T lymphocytes. This effect is likely the result of the elaboration of TH2 cytokine levels, because pulmonary instillation of interleukin-4 or interleukin-13 induces the accumulation of Ear11 transcripts in naive animals. This study demonstrates that despite an allergen-mediated pulmonary eosinophilia and earlier studies showing that Ears are constituents of eosinophil secondary granules, alveolar macrophages are a significant source of these RNases in lungs of OVA-treated mice.

Original languageEnglish (US)
Pages (from-to)678-687
Number of pages10
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume27
Issue number6
StatePublished - Dec 1 2002

Fingerprint

Eosinophil Cationic Protein
Alveolar Macrophages
Ribonucleases
Pneumonia
Genes
Ovalbumin
Gene Expression
Lung
T-cells
Ear
Eosinophil-Derived Neurotoxin
Pulmonary Eosinophilia
T-Lymphocytes
Interleukin-13
Bronchoalveolar Lavage Fluid
Eosinophils
Gene expression
Interleukin-4
Allergens
Animals

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Cormier, S. A., Yuan, S., Crosby, J. R., Protheroe, C. A., Dimina, D. M., Hines, E. M., ... Lee, J. J. (2002). TH2-mediated pulmonary inflammation leads to the differential expression of ribonuclease genes by alveolar macrophages. American Journal of Respiratory Cell and Molecular Biology, 27(6), 678-687.

TH2-mediated pulmonary inflammation leads to the differential expression of ribonuclease genes by alveolar macrophages. / Cormier, Stephania A.; Yuan, Shubing; Crosby, Jeffrey R.; Protheroe, Cheryl A.; Dimina, Dawn M.; Hines, Edith M.; Lee, Nancy A; Lee, James J.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 27, No. 6, 01.12.2002, p. 678-687.

Research output: Contribution to journalArticle

Cormier, Stephania A. ; Yuan, Shubing ; Crosby, Jeffrey R. ; Protheroe, Cheryl A. ; Dimina, Dawn M. ; Hines, Edith M. ; Lee, Nancy A ; Lee, James J. / TH2-mediated pulmonary inflammation leads to the differential expression of ribonuclease genes by alveolar macrophages. In: American Journal of Respiratory Cell and Molecular Biology. 2002 ; Vol. 27, No. 6. pp. 678-687.
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