Truncated hantavirus nucleocapsid proteins for serotyping Hantaan, Seoul, and Dobrava hantavirus infections

K. Araki, K. Yoshimatsu, M. Ogino, Hideki Ebihara, A. Lundkvist, H. Kariwa, I. Takashima, J. Arikawa

Research output: Contribution to journalArticle

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Abstract

Truncated recombinant nucleocapsid proteins (rNPs) of Hantaan virus (HTNV), Seoul virus (SEOV), and Dobrava virus (DOBV) were expressed by a baculovirus system. The truncated rNPs, which lacked 49 (rNP50) or 154 (rNP155) N-terminal amino acids of the NPs of HTNV, SEOV, and DOBV, were able to differentiate HTNV-, SEOV-, and DOBV-specific immune sera. Recombinant NP50s retained higher reactivities than rNP155s and were proven useful for enzyme-linked immunosorbent assay (ELISA). The ELISAs based on the rNP50s of HTNV, SEOV, and DOBV successfully differentiated three groups of patient sera, previously defined by neutralization tests: 17 with HTNV infection, 12 with SEOV infection, and 20 with DOBV infection. The entire rNP of Puumala virus (PUUV) distinguished PUUV infection from the other types of hantavirus infection. Serotyping with these rNP50s can be recommended as a rapid and efficient system for hantavirus diagnosis.

Original languageEnglish (US)
Pages (from-to)2397-2404
Number of pages8
JournalJournal of Clinical Microbiology
Volume39
Issue number7
DOIs
StatePublished - Jul 14 2001
Externally publishedYes

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Seoul virus
Hantavirus Infections
Hantaan virus
Hantavirus
Nucleocapsid Proteins
Serotyping
Virus Diseases
Puumala virus
Viruses
Recombinant Proteins
Enzyme-Linked Immunosorbent Assay
Neutralization Tests
Baculoviridae
Immune Sera
Seoul
Amino Acids
Serum

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Truncated hantavirus nucleocapsid proteins for serotyping Hantaan, Seoul, and Dobrava hantavirus infections. / Araki, K.; Yoshimatsu, K.; Ogino, M.; Ebihara, Hideki; Lundkvist, A.; Kariwa, H.; Takashima, I.; Arikawa, J.

In: Journal of Clinical Microbiology, Vol. 39, No. 7, 14.07.2001, p. 2397-2404.

Research output: Contribution to journalArticle

Araki, K. ; Yoshimatsu, K. ; Ogino, M. ; Ebihara, Hideki ; Lundkvist, A. ; Kariwa, H. ; Takashima, I. ; Arikawa, J. / Truncated hantavirus nucleocapsid proteins for serotyping Hantaan, Seoul, and Dobrava hantavirus infections. In: Journal of Clinical Microbiology. 2001 ; Vol. 39, No. 7. pp. 2397-2404.
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