TRPM1 (Melastatin-1/MLSN1) mRNA expression in Spitz nevi and nodular melanomas

Lori A. Erickson, Gary A. Letts, Sonali M. Shah, Jeffrey B. Shackelton, Lyn M. Duncan

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The transient receptor potential cation channel, subfamily M, member 1 (TRPM1/Melastatin-1/MLSN-1) expression has been shown to have prognostic utility in the evaluation of primary cutaneous melanoma. We analyzed a series of spindled and epithelioid cell nevi (Spitz) and primary cutaneous nodular melanomas to determine whether the expression of TRPM1 mRNA may be useful in distinguishing between Spitz nevi and nodular melanomas and to further examine the patterns of TRPM1 mRNA expression in cutaneous melanocytic proliferations. Formalin-fixed, paraffin-embedded tissues from 95 Spitz nevi and 33 nodular melanomas were analyzed for the expression of TRPM1 mRNA by in situ hybridization using 35 S-labeled riboprobes. Ubiquitous melanocytic expression of TRPM1 mRNA was observed in 56 of 95 (59%) Spitz nevi and 4 of 33 (12%) nodular melanomas. Diffusely scattered loss of TRPM1 mRNA was identified in 38 of 95 (40%) Spitz nevi and 2 of 33 (6%) nodular melanomas. Regional loss of the TRPM1 mRNA expression by a significant subset of dermal tumor cells or a complete absence of TRPM1 expression by the dermal tumor was identified in 27 of 33 (82%) nodular melanomas, but only 1 of 95 (1%) Spitz nevi. These findings suggest that the pattern of TRPM1 mRNA expression may be helpful in the differentiation of Spitz nevi and nodular melanomas. Of the 16 patients who experienced metastasis, 15 (94%) had primary tumors that displayed reduced MLSN mRNA expression by all or a part of the dermal tumor.

Original languageEnglish (US)
Pages (from-to)969-975
Number of pages7
JournalModern Pathology
Volume22
Issue number7
DOIs
StatePublished - Jul 2009
Externally publishedYes

Fingerprint

Epithelioid and Spindle Cell Nevus
Nevi and Melanomas
Melanoma
Messenger RNA
Skin
Neoplasms
Transient Receptor Potential Channels
Epithelioid Cells
Paraffin
Formaldehyde
In Situ Hybridization
Neoplasm Metastasis

Keywords

  • Melanoma
  • Melastatin
  • Prognosis
  • Spitz nevi
  • TRPM1

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Erickson, L. A., Letts, G. A., Shah, S. M., Shackelton, J. B., & Duncan, L. M. (2009). TRPM1 (Melastatin-1/MLSN1) mRNA expression in Spitz nevi and nodular melanomas. Modern Pathology, 22(7), 969-975. https://doi.org/10.1038/modpathol.2009.56

TRPM1 (Melastatin-1/MLSN1) mRNA expression in Spitz nevi and nodular melanomas. / Erickson, Lori A.; Letts, Gary A.; Shah, Sonali M.; Shackelton, Jeffrey B.; Duncan, Lyn M.

In: Modern Pathology, Vol. 22, No. 7, 07.2009, p. 969-975.

Research output: Contribution to journalArticle

Erickson, LA, Letts, GA, Shah, SM, Shackelton, JB & Duncan, LM 2009, 'TRPM1 (Melastatin-1/MLSN1) mRNA expression in Spitz nevi and nodular melanomas', Modern Pathology, vol. 22, no. 7, pp. 969-975. https://doi.org/10.1038/modpathol.2009.56
Erickson, Lori A. ; Letts, Gary A. ; Shah, Sonali M. ; Shackelton, Jeffrey B. ; Duncan, Lyn M. / TRPM1 (Melastatin-1/MLSN1) mRNA expression in Spitz nevi and nodular melanomas. In: Modern Pathology. 2009 ; Vol. 22, No. 7. pp. 969-975.
@article{a1a0e645cd71495cae23c172f6bcb62b,
title = "TRPM1 (Melastatin-1/MLSN1) mRNA expression in Spitz nevi and nodular melanomas",
abstract = "The transient receptor potential cation channel, subfamily M, member 1 (TRPM1/Melastatin-1/MLSN-1) expression has been shown to have prognostic utility in the evaluation of primary cutaneous melanoma. We analyzed a series of spindled and epithelioid cell nevi (Spitz) and primary cutaneous nodular melanomas to determine whether the expression of TRPM1 mRNA may be useful in distinguishing between Spitz nevi and nodular melanomas and to further examine the patterns of TRPM1 mRNA expression in cutaneous melanocytic proliferations. Formalin-fixed, paraffin-embedded tissues from 95 Spitz nevi and 33 nodular melanomas were analyzed for the expression of TRPM1 mRNA by in situ hybridization using 35 S-labeled riboprobes. Ubiquitous melanocytic expression of TRPM1 mRNA was observed in 56 of 95 (59{\%}) Spitz nevi and 4 of 33 (12{\%}) nodular melanomas. Diffusely scattered loss of TRPM1 mRNA was identified in 38 of 95 (40{\%}) Spitz nevi and 2 of 33 (6{\%}) nodular melanomas. Regional loss of the TRPM1 mRNA expression by a significant subset of dermal tumor cells or a complete absence of TRPM1 expression by the dermal tumor was identified in 27 of 33 (82{\%}) nodular melanomas, but only 1 of 95 (1{\%}) Spitz nevi. These findings suggest that the pattern of TRPM1 mRNA expression may be helpful in the differentiation of Spitz nevi and nodular melanomas. Of the 16 patients who experienced metastasis, 15 (94{\%}) had primary tumors that displayed reduced MLSN mRNA expression by all or a part of the dermal tumor.",
keywords = "Melanoma, Melastatin, Prognosis, Spitz nevi, TRPM1",
author = "Erickson, {Lori A.} and Letts, {Gary A.} and Shah, {Sonali M.} and Shackelton, {Jeffrey B.} and Duncan, {Lyn M.}",
year = "2009",
month = "7",
doi = "10.1038/modpathol.2009.56",
language = "English (US)",
volume = "22",
pages = "969--975",
journal = "Modern Pathology",
issn = "0893-3952",
publisher = "Nature Publishing Group",
number = "7",

}

TY - JOUR

T1 - TRPM1 (Melastatin-1/MLSN1) mRNA expression in Spitz nevi and nodular melanomas

AU - Erickson, Lori A.

AU - Letts, Gary A.

AU - Shah, Sonali M.

AU - Shackelton, Jeffrey B.

AU - Duncan, Lyn M.

PY - 2009/7

Y1 - 2009/7

N2 - The transient receptor potential cation channel, subfamily M, member 1 (TRPM1/Melastatin-1/MLSN-1) expression has been shown to have prognostic utility in the evaluation of primary cutaneous melanoma. We analyzed a series of spindled and epithelioid cell nevi (Spitz) and primary cutaneous nodular melanomas to determine whether the expression of TRPM1 mRNA may be useful in distinguishing between Spitz nevi and nodular melanomas and to further examine the patterns of TRPM1 mRNA expression in cutaneous melanocytic proliferations. Formalin-fixed, paraffin-embedded tissues from 95 Spitz nevi and 33 nodular melanomas were analyzed for the expression of TRPM1 mRNA by in situ hybridization using 35 S-labeled riboprobes. Ubiquitous melanocytic expression of TRPM1 mRNA was observed in 56 of 95 (59%) Spitz nevi and 4 of 33 (12%) nodular melanomas. Diffusely scattered loss of TRPM1 mRNA was identified in 38 of 95 (40%) Spitz nevi and 2 of 33 (6%) nodular melanomas. Regional loss of the TRPM1 mRNA expression by a significant subset of dermal tumor cells or a complete absence of TRPM1 expression by the dermal tumor was identified in 27 of 33 (82%) nodular melanomas, but only 1 of 95 (1%) Spitz nevi. These findings suggest that the pattern of TRPM1 mRNA expression may be helpful in the differentiation of Spitz nevi and nodular melanomas. Of the 16 patients who experienced metastasis, 15 (94%) had primary tumors that displayed reduced MLSN mRNA expression by all or a part of the dermal tumor.

AB - The transient receptor potential cation channel, subfamily M, member 1 (TRPM1/Melastatin-1/MLSN-1) expression has been shown to have prognostic utility in the evaluation of primary cutaneous melanoma. We analyzed a series of spindled and epithelioid cell nevi (Spitz) and primary cutaneous nodular melanomas to determine whether the expression of TRPM1 mRNA may be useful in distinguishing between Spitz nevi and nodular melanomas and to further examine the patterns of TRPM1 mRNA expression in cutaneous melanocytic proliferations. Formalin-fixed, paraffin-embedded tissues from 95 Spitz nevi and 33 nodular melanomas were analyzed for the expression of TRPM1 mRNA by in situ hybridization using 35 S-labeled riboprobes. Ubiquitous melanocytic expression of TRPM1 mRNA was observed in 56 of 95 (59%) Spitz nevi and 4 of 33 (12%) nodular melanomas. Diffusely scattered loss of TRPM1 mRNA was identified in 38 of 95 (40%) Spitz nevi and 2 of 33 (6%) nodular melanomas. Regional loss of the TRPM1 mRNA expression by a significant subset of dermal tumor cells or a complete absence of TRPM1 expression by the dermal tumor was identified in 27 of 33 (82%) nodular melanomas, but only 1 of 95 (1%) Spitz nevi. These findings suggest that the pattern of TRPM1 mRNA expression may be helpful in the differentiation of Spitz nevi and nodular melanomas. Of the 16 patients who experienced metastasis, 15 (94%) had primary tumors that displayed reduced MLSN mRNA expression by all or a part of the dermal tumor.

KW - Melanoma

KW - Melastatin

KW - Prognosis

KW - Spitz nevi

KW - TRPM1

UR - http://www.scopus.com/inward/record.url?scp=67650699964&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67650699964&partnerID=8YFLogxK

U2 - 10.1038/modpathol.2009.56

DO - 10.1038/modpathol.2009.56

M3 - Article

VL - 22

SP - 969

EP - 975

JO - Modern Pathology

JF - Modern Pathology

SN - 0893-3952

IS - 7

ER -