Tropism illuminated: Lymphocyte-based pathways blazed by lethal morbillivirus through the host immune system

Veronika Von Messling, Dragana Milosevic, Roberto Cattaneo

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

The immunosuppressive properties of morbilliviruses including measles and canine distemper virus (CDV) are well known, but the host cells supporting infection are poorly characterized. To identify these cells, a recombinant CDV expressing green fluorescent protein was produced by reverse genetics based on a wild-type strain lethal for ferrets. This recombinant virus fully retained virulence and blazed three lymphocyte-based pathways through the immune system of its host: first, it infected rapidly and massively circulating B and T cells; second, it took over and damaged secondary lymphatic organs including spleen, lymph nodes, and gut-associated and mucosal lymphoid tissues; third, it infected most thymocytes. In contrast, replication in epithelial cells was initially not detectable, but substantial before host death. Thus, CDV initially infects lymphocytes and massively replicates therein, thereby causing immunosuppression and preparing systemic invasion and host escape.

Original languageEnglish (US)
Pages (from-to)14216-14221
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number39
DOIs
StatePublished - Sep 28 2004

Fingerprint

Morbillivirus
Canine Distemper Virus
Tropism
Immune System
Lymphocytes
Reverse Genetics
Ferrets
Measles
Lymphoid Tissue
Thymocytes
Immunosuppressive Agents
Green Fluorescent Proteins
Immunosuppression
Virulence
Mucous Membrane
B-Lymphocytes
Spleen
Lymph Nodes
Epithelial Cells
Viruses

Keywords

  • Immunosuppression
  • Measles
  • Virus

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Tropism illuminated : Lymphocyte-based pathways blazed by lethal morbillivirus through the host immune system. / Von Messling, Veronika; Milosevic, Dragana; Cattaneo, Roberto.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 39, 28.09.2004, p. 14216-14221.

Research output: Contribution to journalArticle

@article{928ce1fafaa449de91f692797257b730,
title = "Tropism illuminated: Lymphocyte-based pathways blazed by lethal morbillivirus through the host immune system",
abstract = "The immunosuppressive properties of morbilliviruses including measles and canine distemper virus (CDV) are well known, but the host cells supporting infection are poorly characterized. To identify these cells, a recombinant CDV expressing green fluorescent protein was produced by reverse genetics based on a wild-type strain lethal for ferrets. This recombinant virus fully retained virulence and blazed three lymphocyte-based pathways through the immune system of its host: first, it infected rapidly and massively circulating B and T cells; second, it took over and damaged secondary lymphatic organs including spleen, lymph nodes, and gut-associated and mucosal lymphoid tissues; third, it infected most thymocytes. In contrast, replication in epithelial cells was initially not detectable, but substantial before host death. Thus, CDV initially infects lymphocytes and massively replicates therein, thereby causing immunosuppression and preparing systemic invasion and host escape.",
keywords = "Immunosuppression, Measles, Virus",
author = "{Von Messling}, Veronika and Dragana Milosevic and Roberto Cattaneo",
year = "2004",
month = "9",
day = "28",
doi = "10.1073/pnas.0403597101",
language = "English (US)",
volume = "101",
pages = "14216--14221",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "39",

}

TY - JOUR

T1 - Tropism illuminated

T2 - Lymphocyte-based pathways blazed by lethal morbillivirus through the host immune system

AU - Von Messling, Veronika

AU - Milosevic, Dragana

AU - Cattaneo, Roberto

PY - 2004/9/28

Y1 - 2004/9/28

N2 - The immunosuppressive properties of morbilliviruses including measles and canine distemper virus (CDV) are well known, but the host cells supporting infection are poorly characterized. To identify these cells, a recombinant CDV expressing green fluorescent protein was produced by reverse genetics based on a wild-type strain lethal for ferrets. This recombinant virus fully retained virulence and blazed three lymphocyte-based pathways through the immune system of its host: first, it infected rapidly and massively circulating B and T cells; second, it took over and damaged secondary lymphatic organs including spleen, lymph nodes, and gut-associated and mucosal lymphoid tissues; third, it infected most thymocytes. In contrast, replication in epithelial cells was initially not detectable, but substantial before host death. Thus, CDV initially infects lymphocytes and massively replicates therein, thereby causing immunosuppression and preparing systemic invasion and host escape.

AB - The immunosuppressive properties of morbilliviruses including measles and canine distemper virus (CDV) are well known, but the host cells supporting infection are poorly characterized. To identify these cells, a recombinant CDV expressing green fluorescent protein was produced by reverse genetics based on a wild-type strain lethal for ferrets. This recombinant virus fully retained virulence and blazed three lymphocyte-based pathways through the immune system of its host: first, it infected rapidly and massively circulating B and T cells; second, it took over and damaged secondary lymphatic organs including spleen, lymph nodes, and gut-associated and mucosal lymphoid tissues; third, it infected most thymocytes. In contrast, replication in epithelial cells was initially not detectable, but substantial before host death. Thus, CDV initially infects lymphocytes and massively replicates therein, thereby causing immunosuppression and preparing systemic invasion and host escape.

KW - Immunosuppression

KW - Measles

KW - Virus

UR - http://www.scopus.com/inward/record.url?scp=4644291832&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4644291832&partnerID=8YFLogxK

U2 - 10.1073/pnas.0403597101

DO - 10.1073/pnas.0403597101

M3 - Article

C2 - 15377791

AN - SCOPUS:4644291832

VL - 101

SP - 14216

EP - 14221

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 39

ER -