TrkB kinase activity is critical for recovery of respiratory function after cervical spinal cord hemisection

Carlos Bernardo Mantilla, Sarah M. Greising, Jessica M. Stowe, Wen Zhi Zhan, Gary C Sieck

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Neuroplasticity following spinal cord injury contributes to spontaneous recovery over time. Recent studies highlight the important role of brain-derived neurotrophic factor (BDNF) signaling via the high-affinity tropomyosin-related kinase (Trk) receptor subtype B (TrkB) in recovery of rhythmic diaphragm activity following unilateral spinal hemisection at C2 (C2SH). We hypothesized that TrkB kinase activity is necessary for spontaneous recovery of diaphragm activity post-C2SH. A chemical-genetic approach employing adult male TrkBF616A mice (n. = 49) was used to determine the impact of inhibiting TrkB kinase activity by the phosphoprotein phosphatase 1 inhibitor derivative 1NMPP1 on recovery of ipsilateral hemidiaphragm EMG activity. In mice, C2SH was localized primarily to white matter tracts comprising the lateral funiculus. The extent of damaged spinal cord (~. 27%) was similar regardless of the presence of functional recovery, consistent with spontaneous recovery reflecting neuroplasticity primarily of contralateral spared descending pathways to the phrenic motor pools. Ipsilateral hemidiaphragm EMG activity was verified as absent in all mice at 3. days post-C2SH. By 2. weeks after C2SH, ipsilateral hemidiaphragm EMG activity was present in 39% of vehicle-treated mice compared to 7% of 1NMPP1-treated mice (P. = 0.03). These data support the hypothesis that BDNF/TrkB signaling involving TrkB kinase activity plays a critical role in spontaneous recovery of diaphragm activity following cervical spinal cord injury.

Original languageEnglish (US)
Pages (from-to)190-195
Number of pages6
JournalExperimental Neurology
Volume261
DOIs
StatePublished - 2014

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Recovery of Function
Diaphragm
Phosphotransferases
Neuronal Plasticity
Brain-Derived Neurotrophic Factor
Spinal Cord Injuries
Spinal Cord
Cervical Cord

Keywords

  • Brain-derived neurotrophic factor
  • Diaphragm muscle
  • EMG
  • Spinal cord injury

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience
  • Medicine(all)

Cite this

TrkB kinase activity is critical for recovery of respiratory function after cervical spinal cord hemisection. / Mantilla, Carlos Bernardo; Greising, Sarah M.; Stowe, Jessica M.; Zhan, Wen Zhi; Sieck, Gary C.

In: Experimental Neurology, Vol. 261, 2014, p. 190-195.

Research output: Contribution to journalArticle

Mantilla, Carlos Bernardo ; Greising, Sarah M. ; Stowe, Jessica M. ; Zhan, Wen Zhi ; Sieck, Gary C. / TrkB kinase activity is critical for recovery of respiratory function after cervical spinal cord hemisection. In: Experimental Neurology. 2014 ; Vol. 261. pp. 190-195.
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