Triiodothyronine

a substrate for the thermostable and thermolabile forms of human phenol sulfotransferase

Research output: Contribution to journalArticle

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Abstract

The enzyme(s) responsible for the sulfate conjugation of L-T3 in man has not been characterized. T3 sulfotransferase (T3-ST) activity was characterized in normal human liver tissue obtained during clinically indicated surgical resection. Subcellular distribution studies showed that the T3-ST activity was localized to the cytoplasmic fraction. This finding raised the possibility that T3-ST activity might be similar to the 2 previously identified forms of cytoplasmic phenol sulfotransferase (PST) found in human tissue. A thermostable (TS) form of PST catalyzes the sulfate conjugation of micromolar concentrations of p-nitrophenol, and a thermolabile (TL) form catalyzes the sulfate conjugation of dopamine and other monoamines. Thermal stability and enzyme inhibitor experiments showed that T3-ST activity in pooled liver homogenates was very similar to the TS form of PST. The apparent similarity of T3-ST to TS PST was studied further by measuring T3-ST, TS PST, and TL PST activities in 20 individual liver samples. T3-ST activities correlated significantly with TS PST activities (r = 0.939; P < 0.001) measured with p-nitrophenol, but not with RL PST activities (r = -0.118; P > 0.6) measured with dopamine. However, sulfation of T3 by the TL form of the enzyme might have been masked by the 18-fold higher specific activity of TS than TL PST in human liver homogenates. When the two forms of PST were separated by ion exchange chromatogrphy, T3 was found to be a substrate for both the TS and TL forms of PST. 'True' K(m) values for T3 were similar for TS and TL PST (81 and 127 μM, respectively).

Original languageEnglish (US)
Pages (from-to)1816-1824
Number of pages9
JournalEndocrinology
Volume122
Issue number5
StatePublished - 1988

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Arylsulfotransferase
Triiodothyronine
Sulfotransferases
Sulfates
Liver
Dopamine
Ion Exchange
Enzyme Inhibitors
Enzymes

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Triiodothyronine : a substrate for the thermostable and thermolabile forms of human phenol sulfotransferase. / Young, William Francis; Gorman, C. A.; Weinshilboum, Richard M.

In: Endocrinology, Vol. 122, No. 5, 1988, p. 1816-1824.

Research output: Contribution to journalArticle

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