Trapping of nitric oxide formed during photolysis of sodium nitroprusside in aqueous and lipid phases: an electron spin resonance study.

Ravinder Jit Singh, N. Hogg, F. Neese, J. Joseph, B. Kalyanaraman

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Abstract

Photolytic decomposition of sodium nitroprusside (SNP), a widely used nitrovasodilator, produced nitric oxide (.NO), which was continuously monitored by electron spin resonance (ESR) spectroscopy. The .NO present in the aqueous or the lipid phase was trapped by either a hydrophilic or a hydrophobic nitronyl nitroxide, respectively, to form the corresponding imino nitroxide. The conversion of nitronyl nitroxide to imino nitroxide was monitored by ESR spectrometry. The quantum yield for the generation of .NO from SNP, measured from the rate of decay of nitronyl nitroxide, was 0.201 +/- 0.007 and 0.324 +/- 0.01 (mean +/- SD, n = 3) at 420 nm and 320 nm, respectively. The action spectrum for .NO generation was found to overlap the optical absorption spectrum of SNP closely. A mechanism for the reaction between SNP and nitronyl nitroxide in the presence of light is proposed and computer-aided simulation of this mechanism using published rate constants agreed well with experimental data. The methodology described here may be used to assay .NO production continuously during photoactivation of .NO donors in aqueous and lipid environments. Biological implications of this methodology are discussed.

Original languageEnglish (US)
Pages (from-to)325-330
Number of pages6
JournalPhotochemistry and Photobiology
Volume61
Issue number4
StatePublished - Apr 1995

Fingerprint

Photolysis
Electron Spin Resonance Spectroscopy
Nitroprusside
nitric oxide
Paramagnetic resonance
photolysis
lipids
electron paramagnetic resonance
Nitric Oxide
trapping
sodium
Lipids
methodology
Electron spin resonance spectroscopy
Quantum yield
Computer Simulation
Light absorption
Spectrometry
spectroscopy
optical spectrum

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Trapping of nitric oxide formed during photolysis of sodium nitroprusside in aqueous and lipid phases : an electron spin resonance study. / Singh, Ravinder Jit; Hogg, N.; Neese, F.; Joseph, J.; Kalyanaraman, B.

In: Photochemistry and Photobiology, Vol. 61, No. 4, 04.1995, p. 325-330.

Research output: Contribution to journalArticle

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AU - Joseph, J.

AU - Kalyanaraman, B.

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