Trapping fish genes with transposons

Darius Balciunas, Stephen C Ekker

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Several recent papers describe pilot screens establishing enhancer and gene trap methodologies for use in fish. They have proven these approaches by characterizing genes with novel and sometimes unexpected expression patterns. The resulting fish lines with tissue-specific GFP expression patterns are now being used in further developmental genetics experiments, enhancing the value of fish models for exploring novel biological phenomena. Both Tol2 and Sleeping Beauty transposon systems have been successfully adapted for the construction of enhancer and gene trap vectors. This review summarizes the results presented in these papers and compares this first generation of trap vectors. Future challenges and perspectives for wider use of these methodologies are also discussed.

Original languageEnglish (US)
Pages (from-to)335-341
Number of pages7
JournalZebrafish
Volume1
Issue number4
DOIs
StatePublished - 2005
Externally publishedYes

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transposons
trapping
Fishes
traps
gene
fish
Genes
Biological Phenomena
Beauty
genes
methodology
experiment
tissues
tissue

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Aquatic Science

Cite this

Trapping fish genes with transposons. / Balciunas, Darius; Ekker, Stephen C.

In: Zebrafish, Vol. 1, No. 4, 2005, p. 335-341.

Research output: Contribution to journalArticle

Balciunas, Darius ; Ekker, Stephen C. / Trapping fish genes with transposons. In: Zebrafish. 2005 ; Vol. 1, No. 4. pp. 335-341.
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